Study On The Role And Mechanisation Of NLRP3Inflammasome In Infection Of Microsporum Canis

Objective:Microsporum canis (M.canis)is dermatophyte fungi,a species of genus Microsporun.Usually causes the skin and hair infection. M.canis is zoophilic fungi, frequenly resides in fur of cats and dogs.If human contact with these animal can transmitted to human and leads to tinea corporis and tinea capitis, affect the quality of people’s lives. The number of skin disease caused by M.canis are increasing, because of the most people in city are keeping pets.Host recognition and response to invasion of pathogenic microorganisms highly dependents on the innate immune system. As an important component of innate immunity, the NLRP3inflammasome has been proved to play an important role in response to infection from Candida albicans and other pathogenic fungi, therefore, NLRP3may also be involved in the response to M.canis infection. This study examines the NLRP3inflammasome role in the course of microsporum canis infection, reveal the mechanism of natural immune response of M.canis pathogenesis, and will provide a new molecular targets for the treatment of M.canis infection and related diseases.Methods:To determine whether the inflammasome is involved in host defense against Microsporum canis infection, we irritated human monocytic THP-1cells and mouse dendritic cells with a clinical strain of Microsporum canis isolated from patients with tinea capitis.Use ELISA and Western blotting to test the IL-1β production and caspase-1activation.Results:We found that M. canis infection triggered rapid secretion of IL-1β from both THP-1cells and mouse dendritic cells. our data revealed that M. canis induced production of IL-1β was dependent on the NLRP3inflammasome in vivo. Conclusion:this study unveil that the NLRP3inflammasome exerts a critical role in host innate immune responses against T.sch infection, and suggest that diseases resulted from M.Canis infection might be controlled by regulating the activation of inflammasome.