Screening And Identification Of Differentially Expressed Proteins Among Kerion Strain, Capitis Strain And Corporis Strain Of Microsporum Canis

Objective: Screening and Identification of differentially expressed proteinsamong kerion strain, capitis strain and corporis strain of Microsporum canis. To explorethe pathogenesis of microsporum canis in different sites and infectious degrees.Methods: Choose one strain kerion strain, one capitis strain and one corporisstrain of Microsporum canis, two-dimensional electrophoresis, silver staining, PDquestsoftware analysis, to take the relevant differentially expressed protein gel point, massspectrometry to obtain information about proteins, to analyze and identify through theNCBI database.Results: Kerion protein spots detected is531±20, and capitis protein spotsdetected is265±17, and corporis protein spots detected is161±14. There are more than200different proteins between kerion strain and corporis strain, and the upregulatedproteins is about137in kerion strain. There are more than100different proteinsbetween capitis strain and corporis strain, and the upregulated proteins is about78incapitis strain. There are more than100different proteins between kerion strain andcapitis strain, and the upregulated proteins is about98in kerion strain.Conclusion:1. Two-dimensional electrophoresis of kerion strain, capitis strainand corporis strain from microsporum canis suggest that significant differences doexit on the protein levels, which support that the same fungal may infect different sitesof the body or lead to the same location of different severity.2. Disease related proteins can be inferred through the expressed different proteins.Acquiring meaningful proteins including granzyme A precursor, CDT1protein inkerion strain, and zinc finger protein, beta-tubulin in capitis strain, and hypotheticalprotein in corporis.