Screening Invasion Of Esophageal Cancer Cell Lines Of Different Proteins And Differences14-3-3β Expression And Clinical Significance In Esophageal Squamous Cell Carcinoma

Objective:In this study, the main invasion forces Screening different cell lines of different proteins and14-3-3(3expression in esophageal squamous cell carcinoma and normal esophageal tissues, and to explore differences in protein14-3-3(3relationship between clinicopathological parameters, and prognosis.Methods:In this study, the use of Transwell chamber from esophageal cancer Eca-109cell lines were screened for high invasiveness of esophageal cancer cell lines subline.18O labeled quantitative proteomics using different protein and screened by immunohistochemistry SP method detected78pairs of adjacent tissue microarray esophageal squamous cell carcinoma and its paired in14-3-3β expression.Results:(1) screened Eca-109T0and different metastatic potentials Eca-109T4high invasiveness of esophageal cancer cell lines.(2) the establishment of a stable and efficient18O labeling techniques of isolation and identification of Annexin Al, PRPS1L1, HSP90,14-3-3and other proteins, including14-3-3proteins differentially expressed in more than1.5times.(3) In the next esophageal squamous cell carcinoma and14-3-30expression in the nucleus and cytoplasm were69.2%(54) vs30.8%(24),62.8%(49) vs17.9%(14), which were significantly higher expression in esophageal squamous cell carcinoma adjacent tissues, with statistical significance (P<0.01);14-3-3β with the degree of differentiation of esophageal cancer, a statistically significant (P<0.01), and age, lymph node metastasis, TNM stage, depth of invasion, tumor size (P>0.05).Conclusion:(1) to establish a different metastatic potential of high invasiveness of esophageal cancer cell lines subline will be used to investigate the transfer mechanism of esophageal cancer. (2)18O labeled accurately quantitative and quantitative proteomics to identify differentially expressed proteins esophageal tumor target molecule provides a new screening technology is the technology platform.(3) verification consistent differences in immunohistochemical protein expression levels and8O labeled quantitative proteomics results.(4)14-3-3β expression in esophageal squamous cell carcinoma increases the protein in esophageal squamous cell carcinoma is highly expressed in the nucleus and cytoplasm.(5)14-3-3β positively correlated with tumor differentiation was, the lower the higher the degree of tumor differentiation,14-3-3β expression.(6)14-3-3β with gender, age, TNM stage, tumor size and survival was no correlation.