| Background:Atrial fibrillation (AF) is the most commonly encountered sustained arrhythmia.AF may cause strokes and aggravate heart failures, and is souce of considerablemortality and morbidity. At present, the mechanism of atrial fibrillation are mainlyconcentrated in two aspects of atrial electrical remodeling and structural remodeling.Connexin is an important part of the structure remodeling. Connexin40(Cx40) is akind of Connexin, and is also a new target for the treatment of atrial fibrillation. Inrecent years, there were have been reported few single nucleotidepolymorphisms(SNPs) loci on Cx40gene(GJA5), and some of them were associatedwith AF. Cx40gene rs10465885loci has been found associated with the occurrence ofAF. However, the distribution of SNPs genotype and allele has differences in thedifferent populations and regions, thus further research for rs10465885SNP allele andgenotype distribution and rs10465885associated with AF study in our country, hasthe vital significance.Objectives：1. The purpose of the present study is to investigate the association ofpolymorphisms of Cx40gene with atrial fibrillation.2. Besides, we aim to assess the interaction effect between Cx40gene with coronaryheart disease, hypertension and left atrial diameter, in order to have a bettercomprehension of the pathogenesis for AF.Methods：A case control study was set up, we selected one single nucleotidepolymorphisms(rs10465885) in Cx40gene for association research with AF.Consecutive patients were recruited from1staffiliated hospital of Nanhua university.We collected the venous blood and put it into EDTA tubes. DNA was extracted fromperipheral blood cells by kits. After designed the primers, PCR amplified the purposed bands, checked and purified by Agarose gel electrophoresis. Sequenced by the ABI3070XL sequenator at Beijin Genomics Institute. Finded SNPs by Lasergene software.SPSS13.0statistical software was used. Hardy-Weinberg law of balance was used toutilize the genetic banlance. We utilized testing Χ2to compare count date and testingT to compare measurement data. Unconditional Logistic regression analysisexplored the relationship between gene polymorphism and atrial fibrillation occurred,calculated odds ratio and95%confidence interwal. Standard test α=0.05.Results：1. Atrial fibrillation group enrolled98patients including55males and41females,49cases of hypertension and49of coronary heart disease. Control group enrolled102patients including57males and45females,52cases of hypertension and50of coronary heart disease. AF group and control group were well matched in age,sex, the history of smoke and drink, et al.2. The rs10465885SNP of the Cx40gene was identified by direct sequencing.The frequencies of genotype of AA, AG, GG in AF group was31,44and23respectively, allele frequency A and G was0.54and0.46respectively. Thefrequencies of genotype of AA, AG, GG in control group was18,56and28respectively, allele frequency A and G was0.45and0.55respectively. Thedifference of AA genotype between AF group and control group wassignificant(P=0.02). AG, GG genotype and allele frequency no significantdifference in the two group.3. According to the multi-factor logistic analysis, AA genotype(rs10465885) and theLeft atrial diameter(LAD) were the risk factor of AF. The odds ratios(OR) of AAgenotype was2.49(95%CI:1.02-6.06), which may increase more2.48-fold riskof AF than in those without. the OR of LAD was1.29(95%CI:1.18-1.41), whichmeans per1mm increased in LAD, the risk of having atrial fibrillation in their1.29–fold.Conclusions：1. The rs10465885SNP of GJA5gene existed.2. AA genotype(rs10465885) and LAD are the risk factors of AF. |
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