| AIM To investigate the neuroprotective effect of matrine by activatingcannabinoid2(CB2) receptor on focal cerebral ischemia-reperfusion (I/R)injury in rats.METHODS Focal cerebral I/R injury in rat was made by inserting athread into internal carotid artery for1.5h and then reperfused for24h.Eighty-four male adult SD rats were randomly divided into sham group,I/R group, matrine20mg·kg-1and matrine50mg·kg-1groups,nimodipinegroup,selective CB2receptor antagonist AM630group, andAM630+matrine50mg·kg-1group. The neurological scores and infarctionvolume were detected. HE stain was used to observe the pathologicalchanges. TUNEL was used to measure the apoptosis cells.Immunohistochemistry was used to observe the expression of cleavedcaspase-3and Bcl-2.RESULTS Compared with the model group, the neurological scoresand infarction volume were decreased in matrine20mg·kg-1and matrine50mg·kg-1groups, as well as the pathological changes were relieved, the number of apoptotic cells and cleaved caspase-3expression were decreased,and Bcl-2expression was increased (P<0.05). But these neuroprotectiveeffects of matrine were abolished by pretreatment with AM630, there wassignificant difference between AM630+matrine50mg·kg-1group andmatrine50mg·kg-1group (P<0.01).CONCLUSION The activation of CB2receptor might be involved inmatrine induced neuroprotective effect on focal cerebral I/R injury. |
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