Changes In Glucose-6-phosphate Dehydrogenase Expression Resmlts In Altered Behavior Of HBV-associated Liver Cancer Cells

Hepatocellμlar carcinoma (HCC) is regarded as a global health careissue, and chronic hepatitis B virus (CHB) infection, is considered to beinvolved in pathogenesis of HCC. In order to increase knowledgeof HCC pathogenesis, as well as discover potential novel molecules foranti-cancer therapy, mass spectrometry (MS) and iTRAQ were employed.Samples from nine HBV-related liver cancer patients were analyzed. Intotal,222proteins were analyzed for differential expression in the twotypes of samples. Among these proteins, several were further confirmed byimmunohistochemical, immunoblotting and real-time RT-PCRanalysis. RNA interference induced down-regμlation of G6PD anddecreased HBV replication by5-fold by the IFN pathway. DecreasedG6PD expression resμlted in decreased hepatoma cell migration andinvasion in cell cμlture. In summary, the investigation provides newinformation on pathogenesis of HBV infection, and suggests G6PD as anovel anti-HCC target. G6PD suppression may contribute to treatment strategies for inhibiting tumor progression.