The Expression Of TRX-1in Hepatocellular Cancer And The Correlation With Clinicopathologic Characteristics And Prognosis

Objective:In this study, we detected the expression changes of TRX-1in hepatocellular cancer tissue and tissues surrounding tumors by immunohistochemical SP methods, as well as to correlate with clinicopathological parameters and Overall Survival (OS) in hepatocellular cancer.Materials and Methods:60hepatocellular cancer patients underwent completely resected who were enrolled from the second Xiangya Hospital of Central South University (CSU). Those patients also have complete clinical and follow-up data. Then, tissues surrounding tumors of20cases were selected as controls. Immunohistochemical staining SP method was Performed.Outcome definition of TRX-1was stained Palm yellow particle in cytosol or nucleus, Positive cells<10%as negative,>10%as Positive at high magnification(400x). We use telephone to follow up the Overall Survival Time. Statistical methods Χ2test, Spearman rank correlation test、Kaplan-Meier survival analysis and Cox proportional hazard regression model were performed by SPSS software version17.0.Results:Positive expression of TRX-1in60hepatocellular cancer cases and20tissues surrounding tumors cases was63.33%and15%, respectively. The difference had statistical significance (P<0.01).We can see the positive expression of TRX-1was all stained Palm yellow particle in cytosol or nucleus. Expression of TRX-1was positive correlated with gender、age and negative correlated with expression of tumor size. It had no significant relation to pathology grading、Hepatitis B、cirrhosis. Trx-1expression was negative corrected with overall survival time by Kaplan-Meier survival analysis (P<0.01)。Expression of TRX-1was unique factors effecting prognosis (P<0.05). Gender may influence the prognosis(P=0.076). Age、tumor size、pathology grading、Hepatitis B、 cirrhosis were not unique factor.Conclusions:The expression of TRX-1was higher in carcinoma tissues than tissues surrounding tumors, The difference had statistical significance (P<0.01).Expression of TRX-1was positive correlated with gender、age and negative correlated with expression of tumor size,but not correlated with pathology grading、Hepatitis B、cirrhosis. The patients with TRX-1negative expression had longer Overall Survival than TRX-1positive expression. Detection of TRX-1in hepatocellular cancer tissues might be significant in prognosis prediction. The multiple analysis outcome indicated that despite age、tumor size、pathology grading、 Hepatitis B、cirrhosis were not unique factors for judging prognosis of hepatocellular cancer patients, expression of TRX-1was respectively unique factor. The value of gender approached0.05and possibly correlated with prognosis.