The Expression Of NF-κB In Colorectal Cancer At Different Pathological Staging

[Objective]:To provide a molecular theory for the diagnosis and treatment of colorectal cancer, the relationship between the expression and activities of NF-κB in colorectal cancer tissues and clinicopathological characteristics of colorectal cancer is studied.[Clinical materials and methods]:Between April2013and December2013,78cases of colorectal cancer tissues and the corresponding adjacent normal tissues were collected from patients at Xiangya Hospital during their surgical operations. Expression levels of NF-κB were measured by immunohistochemistry SP method. Activities of NF-κB in tissue samples were also detected. And then how these two characteristics of NF-κB, expression level and activity, were related to gender, age, tumor location, degree of differentiation, depth of tumor invasion, lymph node metastasis, distant metastasis and clinical staging were investigated respectively. All statistical analyses in this study were performed under SPSS19.0, and P<0.05was taken as the significance level.[Results]:For immunohistochemical analysis, in78cases of colorectal cancer,48(61.5%) cases were found NF-κB P65expression positive within cytoplasmics while24(30.8%) cases were observed was NF-κB P65expression positive within nucleus. In18cases with distant metastasis,13had high expression of NF-κB P65within nucleus, accounting for72.2%(13/18); In51cases with lymph node metastasis samples, that number was20, accounting for39.2%(20/51). For activity detection, in78cases of colorectal cancer tissue samples, activity of NF-κB P65was high in30cases, taking up55.6%(30/54) of the total cases.[Conclusion]:Expression of NF-κB might be irrelevant to gender, age,tumor location, but be potencialy relevant to differentiated degree, lymph metastasis, distant metastasis and TNM.