Exploration Of Skin Bacterial Community Between Mother And Infant Through High-Throughput Pyrosequencing

Skin microbes play important roles:they cover the human skin and live on the nutrition produced by human beings, and conversely they will act on skin metabolism; meanwhile, environment will also affect these organisms. Differentiated skin character determined the microbial communityin particular sites. Previously, a basis understanding of skin microbes has formed using culture-dependent method. Deficient of water content, skin microbes are dominated by gram-positive bacteria, while gram-negative bacteria are more abundant in moist sites.Recently, many researchers began to study skin micro flora using16S rRNA gene marker. Rather than identifying some special kind of bacteria, these studies focused on the interaction of skin niches and microbial community. Compared to other niches such as soil or marine, less diverse species are present in skin. These species embrace relative high abundance and exist steadily, which implied that they may be crucial to skin health.This study analyzed mother and infant skin microbial in order to solve several questions:1) Differences among human sebaceous, moist and dry sites;2) Core microbes in mother and infants;3) Relation between infant microbes and outer environment;4) Correlation of microbialcommunities between mother and infant. Conclusion will be drawn combined with previous studies.In this study, skin bacteria are mainly from Actinobacteria, Firmicutes, Proteobacteria and Bacteroidetes. The core member of mother and infant is the similar. However, the abundance varies. There are also some differences in other common residents. The percentage of Propionibacterium is higher in mothers’skin sites than infants’; its growth is high related to sebum. Streptococcusand Staphylococcus are two main organisms in infant skin; moreover, Staphylococcus is more abundant in caesarean birth than vaginal birth. Bacterial members of the infant are closer to his mother, while bacterial structure is more similar to other infants. Most of the unique genera detected in infant are the common oral bacteria, which displays the possibility that the first colonization of human skin microbes may from oral cavity.