Anopheles Sinensis Shanghai Laboratory Populations Build And Relatively Sensitive Baseline To Establish As Well As Its Resistance To Commonly Used Insecticides

Malaria is one of the most important mosquito-borne diseases which serious harm to human health. Malaria widely prevalent in tropical, subtropical and even temperate edge. It is mainly transmitted by Anopheles sinensis in China. The distribution of Anopheles sinensis in China is the vast plain area, in which rice fields planted area is the main or the only medium of malaria. Shanghai belongs to subtropical zone which also is the Anopheles sinensis activities areas. The main control method of Anopheles sinensis based on chemical control, but excessive use of chemical pesticides has led a resistance of insecticides, and the expanding of resistance areas. In order to monitor and prevent the rapid growth of the resistance, it is necessary to establish the distinction doses of insecticides using in Anopheles sinensis. And it will be quickly and easily to monitor the resistance of the field Anopheles sinensis and provide not economic but effective control measures with these basic datas.The experiment established a laboratory susceptible Anopheles sinensis strain, after the observation of the pre-ecological habits and familiar with the Anopheles sinensis husbandry practices; using impregnation method and contact barrel Determination to determine the virulence baseline and distinction between doses of susceptible strains used commonly insecticides sensitive; breeding the establishment of the laboratory susceptible strain (S) for resistance, resistant nurturing by deltamethrin, determinate resistance by impregnation method; distinguish the dose of wild populations of Anopheles sinensis resistant determination. According to the susceptible strain, analyze the level of resistance of insecticides in different regions. The main conclusions are as follows:1. Built populations of Shanghai Anopheles sinensis laboratoryThe Anopheles sinensis population introduced from Parasitic Diseases Control and Prevention, CDC, which reared in the laboratory for more than50years and did not contact with any pesticides. The introduction of the eggs hatched adult mosquitoes as the first generation of this experiment susceptible strain of Anopheles sinensis(S1). Laboratory rearing environment control, as well as the improvement of farming methods, the Anopheles sinensis hatchability, pupation rate and eclosion rate from19.5%in S2generation,41%and72%, respectively, rising to55%of the S12generation,62%maintain the stability and growth of91%, S8-S10generation Anopheles sinensis. The Shanghai Anopheles sinensis laboratory populations build successful.2. Shanghai susceptible strain of Anopheles sinensis sensitive to toxicity baseline and dose-sensitive determination of the larvae and adultsAfter established laboratory susceptible strain of Anopheles sinensis, sensitive virulence baseline determination of larvae to deltamethrin, cypermethrin, permethrin, dichlorvos, BPMC, fenitrothion and temephos evaluate. The results show that the larvae of deltamethrin LC50of0.0086mg/L, sensitive virulence baseline y=13.0586+3.8987x distinguish dose for0.0678mg/L; beta-cypermethrin LC50for0.0101mg/L, sensitive to poison the force baseline for y=9.2950+2.1510x, distinguish dose for0.2432mg/L; permethrin LC50of0.0633mg/L, sensitive virulence baseline y=10.3449+4.4594x distinguish dose for0.4208mg/L; LC50of dichlorvos1.1918mg/L, the sensitive and virulence baseline y=4.3319+8.7669x distinguish dose for4.3914mg/L the; the LC50of Fenobucarb, as0.2709mg/sensitive toxicity baseline for y=11.3541+11.2014x, distinguish dose0.8738mg/L; fenitrothion LC50for0.1150mg/L, the sensitive virulence baseline y=11.5447+6.9681x the distinction the dose to0.4962mg/L; Abate LC50of0.0564mg/L, sensitive toxicity baseline y=10.8033+4.64,66x, distinguish dose to0.357mg/L. Sensitive virulence determination of baseline deltamethrin, cypermethrin, permethrin and BPMC four insecticides on adult mosquitoes. The results for adult mosquitoes to deltamethrin LC50of0.0091mg/L sensitive virulence baseline y=12.5204+3.6871x distinguish dose to0.078mg/L; larvae of beta-cypermethrin LC50of0.0184mg/L, sensitive virulence baseline y=10.2596+3.0291x distinguish dose for0.2152mg/L; larvae permethrin LC50was0.0516mg/L the, sensitive virulence baseline y=8.4266+2.6610x distinguish dose for0.7718mg/L; larvae of BPMC LC50of0.0186mg/L, the baseline for sensitive virulence y=13.8210+5.0963x distinguish dose for0.1064mg/L. Anopheles mosquitoes to distinguish dose recommended by WHO compare, in addition to beta-cypermethrin, deltamethrin, permethrin and BPMC distinguish between the dose of inference basic values close to the WHO.3. Cultivation of resistant strains of Anopheles sinensis results Through12generations of Anopheles sinensis resistant strains cultivation, Anopheles sinensis resistance ratio growth up to3.3times in F12, and the resistance levels has not reached resistance levels. With Lei Xintian, Zhu Changliang the Anopheles sinensis had no resistance generated results consistent with deltamethrin breeding. Resistance to cultivate the same time, to observe the breeding of Anopheles sinensis the larvae pupate rate and emergence rate, along with the development of resistance to the downward trend. The results are consistent with the product-qian, Leixin Tian et al report results.4. In different parts of the population of Anopheles sinensis adult mosquito resistance to insecticides determinationQingpu District, Shanghai, Baoshan District, Jiading District, Jinshan and Chongming County, Hunan province regional collection of wild strains Anopheles sinensis resistant to Deltamethrin, lambdacyhalothrin, permethrin, BPMC and fenitrothion five drugs were evaluated. The results showed that the six regions of Anopheles sinensis are resistant to these five drugs. Anopheles sinensis in Baoshan District reached a high resistance level for five drugs; Qingpu District reached high resistance level to bromine cyhalothrin, permethrin, BPMC Witty; Anopheles sinensis in Chongming County have a high resistance to deltamethrin, initial resistance to beta-cypermethrin; Jiading district of China has initial resistance to beta-cypermethrin and BPMC; Anopheles sinensis in Yongzhou reached a high resistance to deltamethrin, cypermethrin, BPMC and fenitrothion.