Clinical Analysis Of Chromosomal Abnormalities By Fluorescence In Situ Hybridization Detection Of56Cases Of Chronic Lymphocytic Leukemia

Objective:To discuss the relationship between cytogenetics and Rai, Binet staging system in Chronic lymphocytic leukemia, and their prognostic value. Methods:By means of analyzing the karyotype of56patients with chronic lymphocytic leukemia, and applying combined probe to do Fluorescence in situ hybridization detection, to understand the relationship between the chromosomal abnormalities, clinical stage and disease progression, disease prognosis. Results Conventional cytogenetic detection of abnormal karyotype in13(23.2%),by comparing the median overall survival,we found normal karyotype group (>35.9months) and abnormal group had statistically difference (24months)Cχ2=6.60,P<0.05); FISH detected genetic abnormality in38cases (67.9%), abnormal detection rate is significantly higher than the conventional chromosome group (χ2=22.50, P<0.05), RBI was33.9%, D13S25was46.4%, ATM was16.1%, P53was12.5%,involving one kind of gene abnormality was21cases,12cases involving two kinds of genetic abnormalities, involving the three kinds of genetic abnormalities was4cases, involving four genetic abnormality was1case. According to the modified installment Rai, involving genetic abnormality species distribution in the low risk and high risk group had statistically significant(χ2=11.77,P<0.05). With the increase of involvement of the genetic abnormality of the species in the mountain, the incidence of that in high risk group also gradually increased.The median survival of D13S25,RB1normal and abnormal in patients was no difference, respectively,(χ2=0.005, P>0.05) and(χ2=0.032, P>0.05);the patients had P53or ATM gene abnormality that the median overall survival were significantly shorter than normal,13months (χ2-38.76, P<0.05),21months(χ2=4.76, P<0.05), respectively, compared the median survival of patients with not involving genetic abnormality and involving one, two, three, four kinds of gene abnormalities, the patients with involving four gene abnormalities had minimum median survival time. According to the modified installment Rai, the overall survival of lowintermedi-aterisk group was better than that of low risk group (χ2=10.61, P<0.05); according to the modified installment Binet stage, the overall survival of stage C is lower than B (χ2=6.60, P<0.05).Conclusion:The prognosis of patients with the same risk group is very strong heterogeneity, cytogenetics changes combine with clinical stage system are the important prognostic indicators of CLL.