Effects Of Continuous Venovenous Haemodiafltration On Levels Of Cytokine In Dogs With Multiple Organ Dysfunction Syndrome

Objective:To investigate the effects of continuous venovenous haemodiafltration (CVVHDF) on levels of LPS、CD14、HMGB-1and TGF-β in multiple organ dysfunction syndrome (MODS)dogs, and explore the treatment mechanism of CVVHDF.Methods:_Twelve Dogs were subjected to hemorrhasie shock plus resuscitation and endotoxemia to set up MODS model, then they were randomly divided into2groups: MODS group (n=6) and CVVHDF group (n=6). After endotoxin injection complete,the CVVHDF group received CVVHDF treatment for24h; MODS group did not receive CVVHDF treatment. The concentrations of LPS、CDM、HMGB-1and TGF-β in plasma and ultrafiltrate were measured with different time points.Results:Plasma levels of LPS、CD14and HMGB-1were signmcantly decreased in the CVVHDF group at T6、T7、T8and T9after endotoxin injection as compared with MODS group (P<0.01). TGF-β levels were increased in the CVVHDF group at T5、T6、T7、T8and T9after endotoxin injection as compared with MODS group (P<0.01). The sieving coeffcients (SC) for CD14、HMGB-1and TGF-p were0.78±0.10,0.7.±0.09and0.82±0.09.LPS could not be detected in the ultrafiltrate. CVVHDF treatment were significantly reduced the heart and the liver tissue damage. Conclusion:CVVHDF could effectively reduce the levels of pro-inflammatory mediator and anti-inflammatory cytokine related to MODS, rebuild the immune steady-state and protect or gans injured further in the prevention and treatment of MODS.