The Analysis Of Correlation Between Serum IL-17and ALT, AST, TBil In Patients With HCV Infection

Objective:First of all, this research is to discuss the characteristics of changes in different stages of hepatitis C disease development through the detection of concentration levels changes of serum ALT, AST, TBil and cytokine IL-17in hepatitis C patients with cirrhosis, chronic hepatitis C patients and normal control group. Secondly, the correlation between virus load and IL-17, ALT, AST, TBil is discussed by investigating virus load in groups. In addition, the role of IL-17in hepatitis C pathogenesis is investigated by comparing the cytokine IL-17level in patients before and after antiviral therapy.Methods:85hepatitis C patients including24hepatitis C cirrhosis patients and61chronic hepatitis C patients are chosen. Another20hepatitis C patients with more than1×102virus load are chosen for polyethylene glycol interferon combined with Leigh Bhave Lin therapy and30healthy people are chosen as control group. The anti-HCV antibody detection of all the hepatitis C patients shows that S/CO3.8(which means there is no anti-HCV false positive patients). The patients are divided into four groups. The automatic biochemical analyzer is used to test the ALT, AST and TBill level. The ELISA (Enzyme Linked Immunosorbent Assay) method is used to test the serum anti-HCV antibody and cytokine IL-17levels. The RT-PCR (Real Time-Polymerase Chain Reaction) is used to test the HCV-RNA virus load.Results:(1) IL-17levels of all hepatitis C patients are notable higher than healthy control group (P<0.05) and the difference has statistical significance. There is no significant difference between the cirrhosis patients group and the virus load positive group (P>0.05). The ALT, AST and TBil test level has no significant difference between the group with less than1×103HCV RNA test and the healthy control group, but has statistical significant difference(P<0.05) between the group with more than1×103HCV RNA test, the cirrhosis patients group and the healthy control group. The IL-17level has good correlation with ALT, AST and TBil level.(2) According to the division by virus load HCV-RNA level, the ALT, AST and TBil level of each two virus load positive groups have no significant difference. The virus load was positive correlation with IL-17level and the IL-17level increased with the increasing of the virus load.(3) After the antiviral therapy, the levels of ALT, AST and TBil have reduced but the level of IL-17has risen a little. The IL-17, ALT, AST and TBil levels have statistical significant differences before and after antiviral therapy (P<0.05).Conclusion:The high IL-17, ALT, AST and TBil test level of patients group might be related to hepatic cell inflammatory injury and IL-17can reflect the liver injury to some extent with the aggravation of disease. The virus load was positive correlated with IL-17level in the virus test positive patients. After the antiviral therapy, the levels of ALT, AST and TBil have reduced and the inflammatory response of liver cells has eased. The increasing of IL-17level shows that IL-17has involved in the generation process of inflammatory response and plays an important role in the easing process of inflammation. This research has provided a new basis for clinical treatment.