R&D Of Buckwheat Herbal Tea And Its Effect Of Reducing Blood Glocose On Type Ⅱ Diabetes Mice

In China, there is rich culture on herbal tea, which can quickly reduce body heatresistance. Buckwheat is a kind of nutrient-rich food material, which can reduce blood sugar,lower blood fat, lower blood pressure, antioxidant and enhance immunity. Therefore, it hasbecome a trend for comany to combine the strengths of buckwheat and herbal tea to produce anew kind of beverage. This paper mainly studied on the particle size of tea, extraction method,extraction time, the ratio of liquid to solid, soaking temperature and soaking time, which canheavily influence the quality of tea beverage. By studying the dynamic changes of buckwheattea and the component of cold cream, combined with sterilization, there may be a method tosolve the sediment in the beverage. The optimal formulation of buckwheat herbal tea wasdefined Throughsensory evaluation. Its effect on reducing blood glucose on type Ⅱ diabetesmice was investigated. Kunming mice were used as experimental animals. This studyestablished type Ⅱ diabetes model through high fat feed and injection of streptozotocin(STZ). Type Ⅱ diabetes were fed for4weeks with different dose, which contains high-dose,medium-dose and low-dose buckwheat herbal tea beverage. The blood glucose wereevaluated weekly, and Tch, TG, HDL-C, LDL-c, insulin, the antioxidant index of liverpathological slices stained with HE of liver and kidney were evaluated after the mouse dead.Conclusions are as follows:(1) The optimal extraction progress of buckwheat tea was: crush size5s, microwavetreatment3min, liquid-solid ratio40:1, boilled water soaking30min.(2) With the storage, b*,the content of flavonoids and protein in the beverage decreasd,and the content of flavonoids may be decreased to10%. This study showed that producer canadd0.2%β-CD and autoclaving to solve the cold cream.(3) The optimal formula of buckwheat herbal tea beverage was: buckwheat tea: mint:shizhu grass: lemon grass: mangosteen: chrysanthemum=50:1:1:1:1:1.(4) The success ratio of Type Ⅱ diabetes model was61.25%. The result of bloodglucose weekly showed that buckwheat tea and buckwheat herbal tea can lower the glucose ofType Ⅱ diabetes mice, while BG and HBTG had an extremely significant influence(P＜0.01). They can also reduce the insulin in mice and improve the insulin sensitivity. Buckwheattea can increase Tch levels in Type Ⅱ diabetes mice significantly; buckwheat tea and low-dose buckwheat herbal tea can significantly lower HDL-C levels; while others had nosignificant influence on TG, LDL-C levels(P＜0.05).(5) Buckwheat tea had no significant effect on SOD,GSH and MDA, except thatbuckwheat tea can increase the GSH levels(P＜0.05). Pathological slices were observed,showing that buckwheat can protect liver and kidney to some extent. Moreover, BG andHBTG had a better effect than others.