The Effect Of NgR-Rock Signaling Pathway For Impaired RGCs Cultured With High Glucose Concentrations

ObjectiveTo explore the effect of NgR-Rock signaling pathway on the impairment ofretinal ganglion cells（RGCs）.MethodsThe RGCs were cultured in vitro, and then divided into four groups byadding different processing factors: high glucose concentrationgroup(40mmol/L),siNgR group(high glucose concentration medium containingAAV2-siNgR virus),siRNA control group (high glucose medium includingnegative sequence of virus) and the control group. The tested indicators weremeasured at3days after culture.The growth status was observed by invertedmicroscope,the expressions of NgR, Rock and F-actin were detected by usingWestern blot respectively,the cell vitality was determined by MTT method, andthe expression of F-actin and cell morphology were inspected by usingimmunohistochemistry stains.ResultsCompared with the control group, RGCs in high concentration of glucoseand siRNA control groups showed wrinkled volume, lessened process,increased refraction, and upregulated expressions of NgR and Rock, while theexpression of F-actin was decreased, cell viability decreased as well (P<0.05,respectively). In control group and siNgR group, there was no obviousdifference in the expression of NgR, Rock, F-actin and cell vitality (P>0.05,respectively).ConclusionHigh glucose activated NgR-Rock signaling pathways, which played adeleterious effect on the regeneration of RGCs.