| Objective： To explore the related signal transduction mechanism inacid-induced expression of MUC5AC mRNA by airway epithelial cells.Methods：16HBE cells were stimulated by Hydrogen chloride, andtreated with OGR1siRNA and an inhibitors to ERK（PD98059）. Cells weredivided into8groups: a control group（pH7.4）, an acidic stimulation group（pH6.4）, acidic stimulation+OGR1siRNAgroup, pH7.4+OGR1siRNAgroup, acidic stimulation+control siRNA group, pH7.4+control siRNAgroup, acidic stimulation+PD98059group, pH7.4+PD98059group. Thetranscription level of mucin(MUC)5AC was evaluated with reversetranscription-polymerase chain reaction(RT-PCR)．The MUC5AC secretedin supernatant was measured by enzyme-linked immunosorbentassay(ELISA). The expression of OGR1protein and The protein levels ofphosphorylation extracellular signal-regulated kinase (p-ERK) wasmeasured by Western blot.Results：The highest expression of MUC5AC mRNA and MUC5ACprotein in acidic stimulation group compared with the control group （p<0.05）, The expression of OGR1protein and p-ERK were alsosignificantly different from those in control group, The expression ofMUC5AC mRNA and MUC5AC levels of phosphorylation ERK in acidicstimulation+OGR1siRNA group were also significantly decreased, Theexpression of MUC5AC mRNA and MUC5AC protein showed no differentbetween pH7.4+OGR1siRNA and the protein in acidic stimulation+OGR1siRNA group were lower than the acidic stimulation group（p<0.05）, Theprotein control group（p＞0.05）, phosphorylation ERK also showed nosignificant change after transfection of OGR1siRNA, The secretion levelof MUC5AC and the expression of MUC5AC mRNA were much lower inacidic stimulation+PD98059group than the acidic stimulation group（p<0.05）, while there were no significance between the a control groupand pH7.4+PD98059group in secretion level of MUC5AC and expressionof MUC5AC mRNA（p＞0.05）.Conclusion：OGR1/ERK transduction cascade may be upper signalregulators in acid-induced MUC5AC expression in16HBE cell. |
PDF Full Text Request