| Objective:Establish human gastric cancer in nude mice NUGC-4orthotopic xenograft model, to discover the mechanism of ginsenoside Rg3combined5-Fu restraining the metastasis of the human gastric, the influence of the expression of CD133in gastric cancer tissue,and discover the effect of ginsenoside Rg3on hematopoietic.Methods:1.By using the method of in situ transplantation, Establish people gastric cancer NUGC-4tumor strains in BALB/C nude mice back subcutaneous, and observe survival after transplantation tumor mice.2.Completely random methods32only nude mouse model can be divided into four groups, namely:the physiological saline group ginseng saponin Rg3group,5-Fu group and ginseng saponin Rg3+5-Fu group, each group of eight, after orthotopic transplantation9days, tail intravenous saline group was given saline water, each only0.2mL/second, Q2d x15;5-Fu group was given intraperitoneal injection of5-Fu injection, according to the standard20mg/kg,1times a week,3weeks in a row, Rg3group was given ginsenosides Rg3tail intravenous injection, according to the1mg/kg standard,Q2d x15;Rg3ginsenosides+5-Fu group was given intraperitoneal injection of5-Fu injection, according to the standard20mg/kg,1times a week,3weeks in a row, at the same time, according to standard according to1mg/kg, ginsenosides Rg3Q2d x15.3. At the end of the experiment, all animals were sacrificed, open each nude mice, tumor tissue collected in situ, tumor weight was weighed to calculate the inhibition rate, the number of mice in each group to observe distant metastasis, distant metastasis rate calculated for each group.4. Using immunohistochemical method to detect the expression of CD133in tumor tissues.5. After the mice were killed, blood was taken from the eyes, making the blood smear, observe the blood smears of red blood cells, white blood cells and platelets under the high magnification, as well as their shape, arrangement and distribution.Results:1. The human gastric NUGC-4orthotopic transplantation model was established successfully. After modeling since the second week, some nude palpable hard mass in the upper abdomen, mass increases, hard. Orthotopic xenografts100%success rate.2. Determination of tumor weight,and calculat the Inhibition rate,the inhibition rate of Ginsenoside Rg3group,5-Fu group,Rg3+5-Fu group were48.6%,69.2%,78.5%, the weight of xenografts of ginsenoside Rg3group,5-Fu group,Rg3+5-Fu group,were Lower significantly than the saline group,the difference was statistically significant(P<0.05).3.Calculated the distant metastasis of each group. Compared with the saline group,the distant metastasis rate of ginsenoside Rg3group,5-Fu group,Rg3+5-Fu group was significantly lower,the difference was statistically significant (P<0.05);The Rg3+5-Fu group has the lowest rate of distant metastasis.the distant metastasis rate of ginsenoside Rg3group and5-Fu group,also was lower than the saline group.the difference was statistically significant (P<0.05).4.CD133-positive rate of tumor tissues in each group were as follows:saline group86.2%, Rg3group36.7%,5-Fu group38.6%, Rg3+5-Fu group26.5%, compared with the saline group, the other three groups can inhibited the expression of CD133in tumor tissue, the difference was statistically significant (P<0.05), especially Rg3+5-Fu group, it has a significant statistically difference with the saline group.5. Blood smear showed:5-Fu group of white blood cells was significantly reduced, ranging from the size of mature red blood cells, platelets less aggregation. There are a larger number of white blood cells, red blood cell morphology was normal mature platelets piles visible in Rg3group and saline group, there was a significant difference compared with the5-Fu group, but the difference between saline and Rg3group of small, non-statistically significant. Rg3+5-Fu group than in the5-Fu group the number of white blood cells slightly, red blood cell morphology is also more rules, platelet aggregation is also relatively high, the difference was statistically significant.5-Fu on mouse hematopoietic function has some damage, but joint the ginsenosides Rg3can rebound after hematopoietic function in mice, indicating that ginsenoside Rg3on chemotherapy mouse hematopoietic function has a protective effect.Conclusion:l.Nude mouse orthotopic transplantation models was established successfully by using the gastric cancer NUGC-4tumor strains.2.Ginseng saponin Rg3joint the5-Fu can suppress the orthotopic transplantation tumor growth of nude mice significantly,at the same time,it can reduce the distant metastasis rate. Ginseng saponin Rg3joint the5-Fu can reduce the expression of CD133in tumor tissue. And tumor tissues and tumor distant metastasis of CD133positive correlation.3.Ginsenoside Rg3on chemotherapy mouse hematopoietic function has a protective effect. |
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