The Effect Of Down-regulating USP9X By SiRNA On Apoptosis And Viability Of Hepatoma Cells

Objective To investigate the regulation of inhibitingubiquitin-specific protease9x(USP9X) on myeloid cell leukemia-1(Mcl-1)protein expression and its effect on apoptosis and viability of humanhepatocellular carcinoma(HCC)cells SMMC7721and HepG2.Methods There are two groups in the study: USP9X-siRNA groupand NC group.1. The protein expression of USP9X in SMMC7721, HepG2and normal human liver cell line L02at the cellular level was observed byWestern blot;2. SMMC7721and HepG2cells were infected withUSP9X-siRNA, cell apoptosis and cell growth viability were analyzed byflow cytometry and MTT;3. Mcl-1protein,a potential target of USP9X, wasdetected by Western blot after siRNAinterference.Results1.The protein expression of USP9X in SMMC7721andHepG2were both higher than that in L02.2. Compared to NC group,inhibiting expression of USP9X in SMMC7721and HepG2cells obviouslyincreased cell apoptosis and decreased cell viability;3.Compared to NCgroup, inhibiting expression of USP9X in SMMC7721and HepG2cellsobviously suppressed Mcl-1protein expression..Conclusion Expression of USP9X is upregulated in hepatoma cellsSMMC7721and HepG2and down-regulating USP9X by siRNA mayinduce cell apoptosis, inhibit cell growth and down-regulate Mcl-1protein expression in hepatocellular carcinoma cells SMMC7721and HepG2.Thestudy may be helpful to prevention and treatment of HCC.