The Influence Of MiR-7Targeting Proliferation Of Human Gastric Cancer Xenografts In Nude Mice And The Research Of Its Role To The Gene Of EGFR

Objective:To study the effects and mechanism of action targeting EGFR gene of miR-7inhuman gastric cancer xenografts in nude mice, to explore their role in the proliferation ofgastric cancer.Methods:Human gastric cancer cell MGC-803cell lines Culture. Use BABL/c nude mice toestablish an animal model, and the animal model were randomly divided into three groups,the experimental group (transfected with miR-7group within the tumor), negative controlgroup (transfected with intratumoral insignificance of sequence group), control group.miR-7expression can be increased by Lipofectamine2000rpm transient in tumor tissue,and tumor growth were observed. Proving whether the EGFR is the target genes of miR-7by dual luciferase reporter gene assay. Testing mRNA transcription level of EGFR in eachgroup by RT-PCR and protein expression of EGFR by immunohistochenistry respectively.Results:The in vivo transfection of micro-7mimics tumor grow slower than the negativecontrol group and blank control group obviously. RT-PCR detect expression levels of miR-7in every growing tumor group, miR-7expression levels of implanted tumor in miR-7mimics group increased significantly compare with other2groups. Both mRNA and proteinexpression levels in miR-7transfection group are reduced compare with transfection negative control group, transfection reagent group and the untreated group EGFR.Conclusion:1. Tumor growth can be inhibited by promoting expression of gastric cancer growingtumor miR-7, it shows the existence of genes related with tumor proliferation in target genesof miR-7.2. To downgrade the expression of EGFR gene mRNA and protein, MiR–7inhibitsgastric cancer cell growth.This study was supported by the National Natural Science Foundation funding.