Correlation Between Intestinal Flora And Serum Hydrogen Sulfide In Infant With Eczema

Objective:To explore the changes of intestinal flora and serum hydrogen sulfide (H2S) in children with eczema, as well as the correlation between intestinal flora and serum H2S.Methods:1. Research objects and Group of experiment:From August2012to June2013in pediatric outpatient of The First Hospital of Changsha, the case group collected41infants with eczema, who were diagnosed with acute or subacute eczema for the first time. Taking the1:1matched method,41healthy infants matched in age and sex as control group were selected. All the infants did not use any antibiotics and probiotics preparations before two weeks of sampling, the children with infection disease, systemic disease and immunological diseases would be excluded.2. Measurement of the experiment indicators:Through standard plate count method acquired the quantity of Bifidobacterium, Lactobacillus, Escherichia coli and Enterococci in the feces of two group children. Using sensitive sulphur electrode method to determine the content of serum hydrogen sulfide.3. Data statistics:All data are presented as mean±standard deviation (x±S). Paired t test was performed to detect differences of means between the two groups, and Correlation analysis was utilized to study correlations between the intestinal flora and serum H2S. SPSS16.0for Windows statistical packages were used for data analysis. A significant difference was assumed at P<0.05. Result:2. Compared with the control group, infants in case group possessed significantly lower counts of bifidobacteria (t=10.94, P<0.001) and lactobacillus (t=7.84, P<0.001), higher counts of colibacillus (t=6.06, P<0.001) and enterococcus (t=18.56, P<0.001) in their feces.3. The level of serum H2S in case group was significantly lower than the control group (t=7.871, P<0.001).3.The counts of bifidobacteria was positively correlated with serum H2S in both control group and case group,(t=0.471,P<0.001),(r=0.453, P<0.001), respectively.Conclusion The counts of Intestinal flora between two groups had significant differences.Intestinal flora disturbance and the changes of serum hydrogen sulfide were involved in the pathogenesis of infantile eczema, and there existed some correlations between them.1. There were significant differences in intestinal flora between the two groups, with Eolibacillus and Enterococcus predominant in children with eczema, but Bifidobacteria and Lactobacillus predominant in normal children.2. The serum H2S level in children with eczema was significantly lower than normal infants.3. Bifidobacterium and H2S may be involved in the pathogenesis of eczema in infants, with a certain correlation between the intestinal flora and serum H2S level in both control group and case group.