Effect Of Exogenous Hydrogen Sulfide On Intestinal Fibrosis Induced By Trinitrobenzene-sulfonic Acid In Rats

OBJECTIVETo investigate the effect of exogenous hydrogen sulfide on intestinal fibrosis induced by trinitrobenzene-sulfonic acid?TNBS?in rats.METHODSSixty healthy femal Sprague-Dawley rats were randomly divided into 4groups:alcohol-control group,model group,NaHS low-dose and high-dose group.Intestinal fibrosis were induced by TNBS in model group,NaHS low-dose and high-dose group.At the beginning of the third week,low-dose and high-dose NaHS groups were injected intraperitoneally once a day with NaHS?14.4umol/kg and 36.0umol/kg,respectively?,an equal volume of normal saline was injected in alcohol-control and model groups.General situation was observed daily during the experiment.All rats were killed at the end of the fifth week.Serum H₂S level was measured.Their colons were resected for the macroscopic and microscopic scores and collagen area,CollagenI?2?Collagen??TGF-?1?TNF-?were detected by Elisa method,CBS mRNA expression was determined by RT-PCR.RESULTS1)The macroscopic score in model group and NaHS groups was higher thanalcohol-control group.The macroscopic score in NaHS groups was lowerthan model group,while the parameter in high-dose NaHS group wassignificantly lower than low-dose NaHS group?P<0.05?.2)The microscopic score and collagen area in model group and NaHS groupswere higher than alcohol-control group.The microscopic score and collagenarea in NaHS groups were lower than model group,while the parameters inhigh-dose NaHS group were significantly lower than low-dose NaHSgroup?P<0.05?.3)The level of Collagen I?2?Collagen III?TGF-?1?TNF-?in model group andNaHS groups were higher than alcohol-control group.The level ofCollagen I?2?Collagen III?TGF-?1?TNF-?in NaHS groups were lowerthan model group,while the parameters in high-dose NaHS group weresignificantly lower than low-dose NaHS group?P<0.05?.4)The level of CBS mRNA in model group and NaHS groups was lower thanalcohol-control group.The level of CBS mRNA in NaHS groups washigher than model group,while the parameter in high-dose NaHS groupwas significantly higher than low-dose NaHS group?P<0.05?.5)The level of serum H₂S in model group and NaHS groups was lower thanalcohol-control group.The level of serum H₂S in NaHS groups was higherthan model group,while the parameter in high-dose NaHS group wassignificantly higher than low-dose NaHS group?P<0.05?.CONCLUSIONSExogenous hydrogen sulfide was closely related with TNBS induced intestinal fibrosis in rats,and has anti-inflammatory and anti-fibrotic effects within in a dosage—dependent manner.