Effect Of Fisetin On Diabetic Nephropathy And Its’ Mechanism

Objective To investigate the influence of fisetin on the expressionof P300and matrix metalloproteinase2(MMP-2) in the kidney injury ofrats with diabetic nephropathy. Methods Rat models of diabetes wereestablished by injecting intraperitoneally streptozocin. According torandom number table, three groups were divided：normal group，diabeticgroup，and fisetin-treated group. After24weeks，all rats were sacrificed.Biochemical Characters of blood and urine samples were tested．Thepathological changes were observed by paraffin sections staining withHE.The expression of extracellular matrix proteins were analyzed via PASand Masson staining．Location of p300protein expressions were analyzedby immunohistochemistry. The protein expressions of p300and MMP-2were determined by Western blotting. The mRNA expressions of MMP-2were analyzed via real-time PCR. Results The biochemical parameters，and kidney pathological images in fisetin-treated group were better thanthose in diabetic group．The expression of extracellular matrix proteinswere lower than those in diabetic group. Immunohistochemistry analysisshowed that among three groups the expression of p300was mainly inglomeruli, and also expressed in cell nucleus and cytoplasma and the coloration of fisetin-treated group was weakened as compared withdiabetic group. Western blotting analysis showed that the expression ofp300protein in fisetin-treated group was lower than that in diabeticgroup.(P<0．05) The expressions of MMP-2mRNA and MMP-2proteinwere higher than those in diabetic group.（P<0．05） Conclusion Fisetincan attenuate diabetes associated abnormalities in the kidney of rats, whichmay be involved in inhibiting the expressions of p300and enhancing theexpressions of MMP-2.