Exosomes Derived MicroRNA29c Induced Apoptosis Of Bladder Cancer Cells

Objective： To assess whether the bladder cancer cells apoptosis areinduced by exosomes derived microRNA-29c secreted from bladder cancercells by down regulating the expression of BCL-2and MCL-1or not.Methods：28cancer and adjacent tissues were examined byimmunohistochemistry to detect the BCL-2and MCL-1expression. Diseasewas Ta–T1in12patients, T2–T4in16, grade1in8,2in8and3in12. Theexpression of microRNA-29c in cancer tissues was detectes by real-timePCR(QRT-PCR). The adenovirus containing microRNA-29c infectedhuman bladder cancer cell lines BIU-87. MicroRNA-29c in exosomes wasmeasured by QRT-PCR. After BIU-87cells were induced byexosomes-derived microRNA-29c, QRT-PCR was used to detected the levelof microRNA-29c in BIU-87. apoptosis was examined by flow cytometry.BCL-2and MCL-1mRNA expression was detected by reversetranscription-polymerase chain reaction. Western blot was used to determinethe expression of BCL-2and MCL-1.Results: The expression of BCL-2and MCL-1protein was remarkablyincreased in bladder carcinoma (P<0.05), but mainly in the basal and suprabasal layers in adjacent tissues. The expression of microRNA-29c incancer tissues was negatively correlated with the BCL-2and MCL-1,Theexpression level of microRNA-29c in exosomes and BIU-87fromexperiment group was higher than control groups (P<0.05).Exosomes-derived microRNA-29c induced the apoptosis in bladder cancercells (P <0.01). Although only BCL-2was reduced at mRNA level, bothBCL-2and MCL-1was reduced at protein level.Conclusion： The human bladder cancer cells infected bymicroRNA-29c adenovirus can transport microRNA-29c through exosomes,exosomes derived microRNA29c can down regulate the BCL-2and MCL-1protein expression, and induce apoptosis of bladder cancer cells.