The Expression Of NLRP3、IL-18in The Renal Tissue Of IgA Nephropathy Patient And Their Correlation Of Clinicopathological Parameters And MCP-1

ObjectiveTo investigate the expression of NLRP3、IL-18in renal biopsy tissue from IgA nephropathy.In addition,to analyze the NLRP3、IL-18with the association of their clinical and pathology,and with the association of MCP-1, thus exploring their effects in the IgA nephropathy.MethodRenal biopsy specimens from50patients with a clinicopathological.Diagnosis of IgA nephropathy were colleeted,and healthy control group5. The clinical data were collected from the patients,divided into the group of isolated hematuria, simple albuminuria,hematuria and albuminuria. The expression of NLRP3、IL-18and MCP-1were detected with immunohisochemical.The expression levels were semi-quantified by image analysis.Result1.The expression of NLRP3、IL-18and MCP-1in IgA NephropathyThe differences were statisticslly significant between with IgA group and control group. Renal tubules epithelial cells and renal interstitial were showed Positive,Glomerulus was showed weakly.2. The expression of NLRP3、IL-18and MCP-1in IgA Nephropathy with different clinical classification.(1)The expression of NLRP3、IL-18and MCP-1were statisticslly significant between with IgA group and control group.(2) The expression of NLRP3、IL-18and MCP-1were statisticslly significant between witn simple albuminuria group and isolated hematuria group,and significant between witn hematuria and albuminuria group and isolated hematuria group.3. The expression of NLRP3、IL-18and MCP-1in IgA Nephropathy with different pathological type.(1) The differences were statisticslly significant between with FSGS group and MCD group.(2)The expression of NLRP3, IL-18and MCP-1were significantly positively correlated with renal tubular atrophy, renal interstitial inflammation and renal interstitial fibrosis.(P<0.01)4. The relationship between with NLRP3. IL-18and MCP-1.(1)NLRP3、IL-18and MCP-1were significantly correlated with24hour urinary protein Excretion(2)) Correlation analysis showed the expression of NLRP3、IL-18were significantly correlated with MCP-1in clinical classification and pathological type. Conclusion1. In control group,the expression of NLRP3、IL-18and MCP-1were very low,basically did not express,while in IgAN group significant increased, showed that NLRP3、IL-18and MCP-1took part in the occurrence and progress of IgAN.2. The differences were statisticslly significant between with FSGS group and MCD group, indicating the importance of NLRP3、IL-18and MCP-1in pathogenesis of tubulointerstitial damage in IgA nephropathy,they may useful in predicting prognosis of IgA nephropathy.3. The expression of NLRP3、IL18and MCP-1were significantly correlated with24hour urinary protein Excretion, renal tubular atrophy, renal interstitial inflammation and renal interstitial fibrosis, showed that NLRP3、IL-18and MCP-1took part in the occurrence of albuminuria and tubulointerstitial damage.4.The expression of NLRP3、IL-18were significantly correlated with MCP-1,showed that the increase of NLRP3may because the chemotaxis of MCP-1,.the increase of NLRP3,also lead to the raise of IL-18,then take part in occurrence of tubulointerstitial damage in IgA nephropathy.