Association Analysis Of IL-10、IL-18Gene Polymorphisms And Sporadic Parkinson’s Disease In Chinese Han Population

Background:Parkinson’s disease (PD) is a complex neurodegene-rative disease, The mechanism of PD is still not very clear. The pathology feature of it is the degeneration of the dopaminergic neurons in Substantia Nigra and the formation of cytoplasmic eosinophilic inclusion bodies (Lewy body) in dopaminergic neurons in the brain. The loss of dopaminergic neurons is usually associated with glial reaction, peticularly the activation of microglia cells, the conversion of which gives rise to increased expression of cytokines and chemokines. Recently more and more evidence indicated that the neuroinflammatory process may play an important role in the pathogenesis of PD. Studies have found single nucleitide polymorphism(SNP)of cytokine genes located within promoter regions are believe to increase the risk of parkinson’s disease. Non-specific inflammatory factor may associated with cognitive dysfunction and dyskinesia appear in PD.Objective:Detect the prevalence of rs1800871, rs1800872of IL-10gene and rs187238polymorphism of IL-18gene in Chinese PD patients and normal controls, and trying to determine the association between them. And analysis above-mentioned three SNPs may be associated with cognitive dysfunction and levodopa-induced dyskinesias(LID) in PD.PD cognitive dysfunction include Parkinson’s disease dementia(PDD) and Mild cognitive impairment in PD(PD-MCI). Methods:We utilized Time of flight mass spectrometer method to identify genotype of rs1800871, rs1800872of IL-10gene and rs187238of IL-18gene in464sporadic PD (including98cases of PD-LID,100cases of PDD and76cases of PD-MCI) and477healthy controls of the Chinese Han population, using the statistical methods of Chi-square test, and Binary Logistic regression to explore the association between them.Results:1. There is no statistical difference in genotype or allele frequencies of rs1800871, rs1800872, rs187238between sporadic PD patient group and healthy control group (rs1800871genotype:x2=,0.948P=0.622;allele:x2=0.168, P=0.681. rs1800872genotype:x2=1.316, P=0.518;allele:x2=0.363, P=0.547. rsl87238genotype:x2=2.698, P=0.260;allele:x2=0.312, P=0.577)2. There is no statistical difference in genotype or allele frequencies of rs1800871, rs1800872, rs187238between PDD, PD-MCI and PD patients with non-cognitive dysfunction.3. There is no statistical difference in genotype or allele frequencies of rs1800871, rs1800872, rs187238between PD-LID and non-LID in PD patients.Conclusion:1. IL-10gene promoter rs1800871, rs1800872polymorphisms and IL-18gene polymorphisms rs187238are not associated with sporadic PD in ethnic Chinese population.2.Above-mentiond three SNPs are not associated with cognitive dysfunction and dyskinesia in PD patients.