The Affect Of Sodium Nitroprusside On Microvascular Blood Flow Of Vital Organs In A Rat Model After Cardiopulmonary Resuscitation

Object:observing the affect of Sodium nitroprusside on microvascular blood flow of vital organs in a rat model post-cardiopulmonary resuscitation (CPR), in order to find new ways for improving microcirculation perfusion after cardiopulmonary resuscitation (CPR) in rats。Methods:SD rats as experimental subjects, after anesthetizing, fixing, endotracheal intubating and catheterizating of femoral artery and femoral vein, the rats were randomly divided into the sham group (n=8)、control group (n=8) and experimental group (n=8)。Sham group: thoracotomy at the beginning of the experiment and injected colored microspheres into the left ventricle at the time of Omin,15min and40min. Control group and the experimental group was stimulated epicardial by electrical to establish cardiac arrest model, and cardiopulmonary resuscitation was carried out to achieve return of spontaneous circulation, The control group was infused normal saline4ml per hour and5%glucose solution2ml per hour15min after the restoration of spontaneous circulation. Experimental group was infused normal saline4ml per hour and5%glucose solution2ml per hour which soluted sodium nitroprusside15min after restoration of spontaneous circulation (SNP concentration of0.01mg/ml). Before the cause fibrillation,15min and40min after the restoration of spontaneous circulation,experimental group and control group were thoracotomyed and injected colored microspheres into the left ventricle.5min after the third microsphere was injected,rats were killed and heart brain, kidney, stomach, intestines, lungs of the rats was get,tissue was digested and microspheres was recovered to calculate the microcirculatory blood flow in vital organs.Results:1、The number of the microspheres in per gram tissue at three time points of Sham group have no significant differences (P>0.05).2、In the control group and the experimental group,compared with the before fibrillation time,the microspheres in per gram tissue of Heart, brain, kidney, stomach, intestines have significantly decreased at the time of ROSC15min,and the microspheres in per gram tissue of lung have significantly increased(P<0.05).3、In the control group, Compared to the time of ROSC15min, the microspheres in per gram tissue of Heart, brain, kidney, stomach, intestines have no significantly differences at the time of ROSC40min(P>0.05).In the the experimental group, Compared to the time of ROSC15min, the microspheres in per gram tissue of Heart, brain, kidney, stomach, intestines have significantly increased at the time of ROSC40min (P<0.05), and the microspheres in per gram tissue of lung have significantly decreased (P<0.05)Conclusions:1、After cardiac arrest,when rats reach restoration of spontaneous circulation, microcirculation blood flow of heart, brain, kidney, stomach, intestinal was decreased.2、Using sodium nitroprusside can improve the microcirculation blood flow of vital organs in rats after cardiopulmonary resuscitation40minutes.