Observation And Clinical Significant Of NF-κB Activity In Peripheral Blood Mononuclear During Cardiopulmonary Resuscitation

Objective To explore the relation between the level of NF-kB in peripheral blood lymphocyte and prognosis of cardiopulmonary resuscitation (CPR).Methods 60 patients with sudden cardiac arrest received CPR. Patients were divided into 3 groups. The patients who didn't regain heart beats were group A, those 21 who kept blood circulation for over 2 hours but finally died were group B, and the remaining 10 who survived were group C. Control group ( group D) was made up of 20 people with no disease. NF-kB was studied by flow cytometry (FCM) technique using immuno-fluorescent staining. The expression of NF-kB activation in peripheral blood lymphocyte was determined at the time of 0, 1, 3, 6, 12h after CPR in all patients.Results The expression of NF-kB activation was no significant difference at the beginning of CPR in all groups. In group C, it began to increase at 1h point time, and it's peak was at 6h, then decreased and wasnear to the normal level after 12h. No significant difference was found before 3h in group B and C. After that, the NF-kB activity significantly increased in 6h, with a peak during 6h~12h.Conclusions The expression of NF-kB activation participate in the onset and development of whole body ischemia-reperfusion of CPR. In the early stage of CPR, NF-kB may has protective effects to the body. But the massive expression of NF-kB activation is associated with high mortality of patients with CPR.