| Objective:To study the effect of high temperature stimulation on the diameter of blood vessels and the vasodilators against the effect.Materials and methods:Adopting controllable temperature electric welding machine simulating physical effect of the heat of the high-frequency electrosurgical generator, to form local high temperature stimulate on the outer wall of the blood vessels.Use it to be the high temperature stimulation on blood vessels animal model. The first phase: Using24adult Wistar rats, both male and female, weight (250-300g). Animals were randomly divided into three groups, each group of eight,exposed common carotid arteries, choose at normal room temperature,50to60℃,90-100℃three temperature points to stimulate the left common carotid artery.After that, cut out the blood vessels which have been stimulated immediately, make it into paraffin section. After HE dyeing, then measure the perimeter, area, diameter and other data of the blood vessels by light microscope. The above data after SPSS19.0processing, choose one of the most statistically significant group, using the group temperature as stimulus, to enter the next step. The second phase:Using24adult wistar rats, both male and female, weight (250-300g). Animals were randomly divided into three groups, each group of eight,exposed common carotid arteries, choose at temperature of90-100℃to stimulate the left common carotid artery. Three groups, respectively, using three kinds of following drugs as drug intervention: saline solution (As a comparison),nimodipine,papaverine. Cut out the blood vessels which have been stimulated after ten minutes, make it into paraffin section. After HE dyeing, then measure the perimeter, area, diameter and other data of the blood vessels by Light microscope. Use SPSS19.0to process the data above.Results:HE staining observed:(1) The first phase:①The normal room temperature group:Blood vessels form did not see obvious difference on both sides, most vascular lumen of them are plentiful, vascular wall form is regular, The structural integrity of the outer wall and endothelial cells is wonderful. Cells arranged closely. Smooth muscle cell in the medial membrane arranged appropriately.②50-60℃group: Most of left blood vessels form become deformed, the degree is medinm.most vascular lumen of them are plentiful, vascular wall form is regular, The structural integrity of the outer wall and endothelial cells is good.Cells arranged closely. Smooth muscle cell in the medial membrane arranged appropriately.The form of right blood vessels is the same with normal temperature group.③90-100℃group:Left blood vessels form become deformed,some of them are seriously. The structural integrity of the outer wall cells is almost complete. Smooth muscle cell in the medial membrane arranged disorder. Endothelial cells arranged closely. The form of right blood vessels is the same with normal temperature group.(2) The second phase:Right blood vessels of three groups are no obvious different with each other.Left blood vessels are different:①Saline solution group:Blood vessels form become deformed,some of them are seriously. The structural integrity of the outer wall cells is almost complete. Smooth muscle cell in the medial membrane arranged disorder. Endothelial cells arranged closely.②Nimodipine group:Blood vessels form become deformed,some of them are seriously. The structural integrity of the outer wall cells is almost complete. Smooth muscle cell in the medial membrane arranged disorder. Endothelial cells arranged closely.③Papaverine group:Most of left blood vessels form become deformed, the degree is medinm.most vascular lumen of them are plentiful, vascular wall form is regular, The structural integrity of the outer wall and endothelial cells is good.Cells arranged closely. Smooth muscle cell in the medial membrane arranged appropriately.Results of statistical analysis:(1) The first phase:All diameter of blood vessels between the three groups are no statistically difference before stimulated.After the high temperature stimulation on left blood vessels:①In every group:T test results between both side of blood vessels’diameter statistically significant difference (P<0.05) in the 90-100℃group and50-60℃group. Besides,90-100℃group is more singificant than50-60℃group(50-60℃group0.01<P<0.05,90-100℃group P<0.01). There were no statistical differences between both side of blood vessels’diameter in the normal room temperature group (P>0.05).(2)Comparing with three groups each other:SNK-q test results show that there were no statistical differences between the normal room temperature group and50-60℃group(P>0.05).90-100℃group has the between the significant statistically difference with the other two groups(P<0.05).It means the high temperature(50-100℃) stimulation on blood vessels can cause vasospasm,and positively related to the degree of temperature.(2) The second phase:①Right blood vessels(Not been stimulated):There were no statistical differences between three groups (Kruskal-Wallis test, P>0.05).②Left blood vessels(Have been stimulated):There were no statistical differences between saline solution group and nimodipine group (P>0.05). There were no statistical differences between nimodipine group and papaverine group (P>0.05). SNK-q test results between saline solution group and papaverine group statistically significant difference (P<0.05).Relationship of three groups’mean value: saline solution group<nimodipine group<papaverine group. It means papaverine has a confrontation effect to vasospasm which caused by high temperature stimulation,but nimodipine do not. Conclusion:1. The high temperature (50-100℃) stimulation on blood vessels can cause vasospasm,and positively related to the degree of temperature.2. Nimodipine do not has a confrontation effect to vasospasm which caused by high temperature stimulation.3. Papaverine has a confrontation effect to vasospasm which caused by high temperature stimulation, the mechanism at the molecular level is not clear yet,maybe apply to relaxe smooth muscles by accelerate update of the intracellular cAMP and cGMP, because papaverine could induce the activity of phosphodiesterase. The further research needs to be continued. |
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