The Structure And Function Prediction Of Multiple Myeloma Tumor Associated Gene Clorf35by Bioinformatics

Abstract:Multiple myeloma (MM) is a hematological malignancy characterized by the uncontrolled proliferation of monoclonal plasma cells. MM is common in old people. Because of the chromosome translocation, MM cells showed different number and structure abnormalities of some chromosomes.Clorf35gene is a novel multiple myeloma tumor-associated gene. The studies showed that Clorf35can lead to cell transformation and promote cell proliferation. In addition, Clorf35cause tumorigenic ability in nude mice experiments. But, how does Clorf35function in MM pathogenesis is still unknown.In this research, by using RT-PCR, we compared the expression levels of Clorf35gene in MM samples and health groups. At the same time, by analyzing the DNA, RNA and protein structures of Clorf35, we predicted the interaction proteins and functional domains of Clorf35, and possible signal transduction pathways taking part in by Clorf35. These would provide evidences for further research on the important function of Clorf35.The results showed that Clorf35is distinctly overexpressed in MM samples (21/29). Bioinformatics analysis showed the encoded products of Clorf35are located at cell nuclus. Clorf35is a kinase phosphorylation protein, there is a glycine-rich domain that is the most highly conserved region of a family of proteins that in vertebrates are associated with tumors in MM. The region may contain phosphorylation sites for several protein kinases, as well as N-myristoylation sites and nuclear localization signals, so it might act as a signal molecule in the nucleus. The interaction protein of Clorf35protein includes THAP1, which is associated with regulating cell cycle progress. This suggests Clorf35regulate cell growth and proliferation by many pathways.