| ObjectiveTo observe the effects of novaferon on the expression of MCP-land IL-8in murine intestinal mucosa induced by DSS with experimental colitis.Methods70specific pathogen free, about8week-old female BALB/c mice (weight18-22g), were randomly divided into7groups:normal, model, mesalazine control, prednisone control, novaferon low-dose,novaferon middle-dose, novaferon high-dose group.10mice were involved in each group. The normal group-mice were fed with distilled water. Intervention group-mice were fed with4%dextran sulfate sodium (DSS) freely for7continuous days to complete ulcerative colitis model. At the same time, every experimental group was orally administrated physiological-saline, physiological saline, mesalazine, prednisone, novaferon0.1ug/ml、0.2ug/ml、0.3ug/ml intraperitoneal injection respectively. During the experiment, general situation, daily weight, stool trait,disease activity index(DAI)and occult blood were recorded,and mice were killed on the8th day, observed the histological scores. Immunohistochemistry (IHC) was used to measure the expression of MCP-1and IL-8in colonic mucosa.Results1.BALB/c mice treated with DSS solution developed diarrhea, mucous stool and bloody stool. With days,DAI scores of all Intervention group were increasing. Novaferon, mesalazine, prednisone can improve the general situation of the mice.Compared with model group, the DAI scores and histological scores were decreased.2.In mesalazine control,prednisone control and novaferon groups,the expressions of MCP-1and IL-8were both decreased, compared with model group(P<0.05)ConclusionThe manifestations and histologic characteristics of DSS-induced acute colitis mice were similar to UC patients. Novaferon, mesalazine,prednisone can decrease the expression of MCP-1and IL-8in murine intestinal mucosa. |
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