| Background and ObjectAnti-N-methyl-D-aspartate Receptor Encephalitis is a kind of autoimmuneinfectious disease on of central nervous system, which may has ovaries teratogenictumors under the paraneoplastic disease category. In the prodromal period of it in theclinical context, along with persistent irregular fever and headache, flu-like symptomsmay emerge. And some obvious psychiatric symptoms could be presented, such asanxiety, agitation, bizarre behavior, hallucinations, delusions, confusion, insomnia,anorexia or language barriers and excessive ingestion. In addition, the possibility ofmemory deterioration is so large, as well as low level of consciousness, epilepticseizures and dyskinesia and the dysfunction of autonomic nervous. The specificity of"δ brush” appears in the EEG, and specificity antibodies of the disease can be testedin cerebrospinal fluid or serum anti-NMDAR antibody. The onset of Anti-NMDARencephalitis can be at any age and more common in children and women. Theaverage age of women with it is23years old. Besides, the detection of ovarianteratoma is closely related to age,56%of18-year-old or older women with a singlelateral or bilateral ovarian teratoma and less than9%of the14year-old or youngergirls with it. According to the clinical data, the early treatment is effective, including early diagnosis, immunosuppressive therapy and removal of tumor from the patients.But the pathogenesis of encephalitis is still unclear and can’t be tested by some givenprinciples currently. What’s more, its manifestation features the obvious individualdifference, difficult diagnoses, wide range of clinical symptoms and potentially lethalrisks. Therefore, further research is needed to find a high sensitivity diagnosticmarker with a high stability and accuracy.MicroRNAs (miRNAs) are a class of highly conserved non-coding dxRNAmolecules, about22nucleotides in length, leading to the degradation of the targetmRNA or translational inhibition by pairing with a target gene3’(3’. UTR)incomplete base. Therefore, it plays a negative role in the expression of eukaryoticgenes. The circulating MicroRNA is wrapped by secretes, microbody or apoptosisbody which effectively protect it. In addition, the MicroRNA having resistance toRNase for the combination of chemical modification of MicroRNA, lipid and proteinmolecules, which indicate MicroRNAs become a potential diagnostic marker.Therefore, to investigate the different expression and change of MicroRNA in theanti-assisted anti-NMDAR encephalitis the diagnostic differences in the patients’plasma is easy to find out MicroRNA with statistical significance. And following themain clue of the research, the potential diagnostic marker could be found, as anassistor to test anti-NMDAR.MethodThe experiment has two steps. Step One: Using microarray gene chip technologyto detect expression level of MicroRNAs in plasma between anti-NMDARencephalitis patients and healthy human. Then, with statistical significance, thedifferential expression of MicroRNA could be screened out. Step two: Basing onmicroarray results and literature, the MicroRNA-140is selected as evaluationparameters； then we collect the plama in different intracranial infection patientswhich is divided into four groups: tubercular meningitis group, viral encephalitis ormeningitis group, healthy group and anti-NMDAR encephalitis group. Then,expression levels of MicroRNA-140in plasma of each group are detected by thereal-time quantization of PCR Result1By chip results,140differentially expressed miRNAs genes are screened outwhich associates with anti-NMDAR encephalitis (p <0.01):53up-regulationexpressions and87down-regulation expressions. Among them, the expression ofMicroRNA-140downgrade more than five times in anti-NMDAR encephalitis (|log2|=5.11, P=0.004).2The expression level of MicroRNA-140in patients’ Plasma with anti-NMDARencephalitis is significantly lower than tuberculous brain inflammation group, viralbrain inflammation group, healthy group (P <0.05). Their down-regulation differenceof MicroRNA-140in plasma is not statistically significant (P>0.05), compared tothat of tuberculosis brain inflammation group, viral brain inflammation patient groupand the healthy group.Conclusion1.The obvious decline of MicroRNA-140in the plasma of patients withanti-NMDAR encephalitis.2. The specific expression changes of MicroRNA-140in the plasma of theencephalitis may be the potential diagnostic biomarkers as auxiliary NMDARencephalitis diagnosis. |
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