TNF-a Expression Differences And Their Significance In Simple Leg Pain And Back Pain Of The Intervertebral Disc In Merger

BackgroundLumbocrural pain is a common disease and frequently occurring in everyday,studies have shown that more than85%of the people in his life to some extentaffected by lumbocrural pain and lumbar disc is the most common etiology oflumbocrural pain is generally believed that at present, joints and ligaments of thedegeneration of intervertebral disc degeneration, caused by the consequences ofintervertebral disc metamorphosis is often disease of lumbar disc herniation is acommon and frequently-occurring disease of bone that is caused by the waist and/orleg pain the most common reason for a large number of literature reports, causing thesymptoms in addition to the mechanical compression, inflammatory cytokines inlumbar degenerative disease also play an extremely important role, for example theTNF alpha nitric oxide interleukin8interleukin1interleukin6research ofprostaglandin E2and so on.ObjectiveLumbar disc caused by lumbar pain with lower limb pain and simple lower limbpain, respectively to compare the two groups of cases of fiber ring in the nucleuspulposus TNF-a positive expression differences have statistical significance.MethodsCollect the first affiliated hospital of zhengzhou university spine treated, needoperation treatment of patients with simple lumbar disc annulus fibrosus and nucleus pulposus tissue, as experimental group,40cases of low back pain (VAS pain scoregreater than4points) merger of20patients with lower limb pain simple lower limbpain (VAS pain score greater than7points)20cases (24male, female16cases, withan average age of42years old from L3~43cases,18cases of L4~5,19cases ofsurgical excision specimens of intervertebral disc L5~S1made paraffin section.Operations were done by the same group of physicians, after removing three timeswith isotonic sterile saline, separating the annulus and nucleus pulposus, paraffinarchive backup. After the collection is completed using immunohistochemicalstaining for two specimens annulus and nucleus pulposus were measured, to observethe expression of TNF-a is positive and the positive intensity of samples in eachgroup.ResultsImmunohistochemical staining: in the two groups of different cases, all of the fiberring specimen were measured to TNF-a, its expression intensity has strong and weakpoints, the t test, fiber ring TNF-a positive expression of integral (t=1.964, P=0.065>0.05), compared two groups have no obvious difference between specimensof nucleus pulposus, expressed as part of TNF-a positive, some negative, throughstatistical analysis and qualitative analysis, two groups of TNF-a qualitativeexpression in the nucleus pulposus (X=1.904, P>1.904), compared two groups, alsohave no obvious difference.Conclusion1. The metamorphosis of lumbar disc is outstanding, can cause proinflammatoryfactor TNF-a large increase;2. Simple lower limb pain caused by lumbar disc and merge back pain in the twogroups of patients, the highlight of the intervertebral disc tissue TNF-a there was nosignificant difference to express meaning.