Effect Of Saxagliptin On The Intestinal Flora And The Chronic Inflammation Status In Patients With Type2Diabetes Mellitus

Type2diabetes mellitus (T2DM) has become the major social and health problem of the world, but the pathogenesis and mechanisms are still not completely understood. T2DM results from interactions between genetic and environmental factors, intestinal flora is closely related to T2DM.In recent years, more and more data have been confirmed that the occurrence of intestinal flora and obesity, T2DM and other metabolic diseases were closely related, resulting in a lot of new research ideas and groundbreaking research, also providing important clues for the understanding of mechanisms and the search for new therapeutic targets. Studies have shown that many patients appear the phenomenon of alteration of intestinal flora, and they have certain relevance. Normally, a dynamic ecological existed among intestinal flora、 host and the external environment，which plays an important role in maintaining the human health. Intestinal flora involved in the regulation of energy metabolism and non-specific immune function, it can affect the immune system, leading to inflammation. The insulin signaling transduction pathway may be impaired by the secretion of cytokines, such as LPS、C-reactive protein，then leading to insulin resistance(IR) and T2DM.Now it is widely believed that T2DM is a chronic low-level inflammation. Therefore, to correct the intestinal flora and improving the chronic inflammation may become a new target for the treatment of IR and T2DM.But there has rare reports on the influence of hypoglycemic drugs, then we perform an experiment to study the effect of Saxagliptin on intestinal flora and the chronic inflammation status in patients with type2diabetes mellitus.ObjectiveTo study the effect of Saxagliptin on intestinal flora and the chronic inflammation status in patients with type2diabetes mellitus.Methods1.10cases of type two diabetes mellitus patients were given5mg Saxagliptin once a day for two weeks. The feces of the patients were collected, before and after taking the medicine. The feces of10healthy persons were also collected as controls.2. Then we extracted the genomic DNA of fecal flora, and Enterobacterial repetitive intergenic consensus sequence polymerase chain reaction(ERIC-PCR) was used to set up the DNA fingerprint of intestinal flora, made agarose gel electrophoresis, then put the gel under UV lamp to image and saved the frigerprint.3. Monitor the blood glucose level of type2diabetes mellitus patients regularly, and the blood glucose level, blood lipid, inflammatory factors of the peripheral blood samples were also tested, before and after taking the medicine.4. Statistical analysisThe data analyzed with SPSS17.0statistical software were showed as (x±s). T test was used in compare between two groups. α=0.05was choose as test level. P<0.05was considered as significant.5. Analysis of the DNA fingerprintUVP gel electrophoresis analysis software was used to assess the similarity and diversity among these groups which were before taking the medicine、after taking the medicine and normal controls.ResultsAfter the treatment of2weeks，the intestinal dysbacteriosis of diabetes was improved，the levels of blood glucose and inflammatory factor were lower than before(P<0.05)，the level of blood lipid had no significant difference than before (P>0.05).ConclusionsSaxagliptin had benefits on improving the intestinal dysbacteriosis in patients with type2diabetes mellitus，and it also could improve the chronic inflammation status.