| spinal cord injury (SCI) is a kind of serious and comprehensive damage of thecentral nervous system, characterized by the loss of sensory and motor functionbelow the level of injury, incontinence, and etc. SCI exerts heavy burden onfamilies as well as the society as a whole, therefore, the researches on the interventionand treatment of SCI are urgent. It is generally believed that, the complexpathological mechanism is the root cause of the difficulty in treating SCI. Stem cellsare cells, which have self-renewal and multiple differentiation potential. Currently,stem cells, which have unique properties and special effects on intractable diseases,provide new opportunity for the treatment of SCI. Since In’t Anker et. showed thatWJCs derived from the umbilical cord have similar immune phenotype to stem cellsderived from bone marrow, and better capacity of prolification, WJCs derived fromthe umbilical cord have attracted more attention. Our preliminary results haveindicated that WJCs have neuroprotective effect on rats with spinal cord injury, and itcan promote recovery of the motor function. However, the molecular mechanisms ofWJCs in the treatment of SCI are unclear. Therefore, this study aims to investigate thetherapeutical effects of transplanting mesenchymal stromal cells derived fromWharton ’s Jelly cells’ on rats of spinal cord injury and the underlying mechanisms.Methods1. WJCs were isolated and cultured in vitro by tissue explants adherent methods,and the specific cellular surface marker were detected by flow cytometry.2.We established the model of spinal cord injury in rats by hemisection in spinalcord. There are12in the Sham-operated group and60rats in the operated group. Thelatter group was then randomly divided into the control group and the transplantationgroup, in which WJCs (200)il,1x10^) were injected by caudal vein10minutes atferinjury. There were10time points(Oh、3h、6h、12h、Id、3d、7d、14d、21d mid28d)in each group,3rats at a time (6rats inl d,3d and7d).3.Motor improvement of the rats were accessed by Basso-Beattie-BresnehanScore1d,3d,7d,14d,21d and28d after WJCs transplantation respectively.4.Histomorphological changes in the injured spinal cord were observed bytransmission electron microscope and immunohistochemistry at28d atfertransplantation.5.The mRNA expression of TNF-a,MCP-land IL-10of the injured spinal cordwere detected by real-time quantitative PCR at0h,3h,6h,12h,24h,72h and168hatfer transplantation,and GFAP, NSE and BDNF at28d atfer transplantation.6.The protein expression of TNF-a,MCP-land IL-10of the injured spinal cordwere detected by Western blot at0h，3h，6h,12h,24h,72h and168h aftertransplantation,and GFAP, NSE and BDNF at28d atfer transplantation.Results1.Motor function were improved in both the control group and the WJCstransplantation group, and the latter was improved more significantly (P<0.05).2.Compared with the control group, the structural of the lesion in spinal cordwas more integrate, and connection was established in axon center in the WJCstransplantation group. The mRNA and protein expression of MCP-1,TNF-aand IL-10of the injury spinal cord in both the WJCs transplantation group and the control groupwere higher compared with the Sham-operated group(p<0.05). The expression ofMCP-1and TNF-ain the WJCs transplantation group were lower than those in thecontrol group (p<0.05), while the expression of IL-10was contrary(p<0.05).3.Comparedwith the control group, the mRNA and protein expression of GFAP, NSE and BDNF of the injury spinal cord in the group of WJCs transplantation at28datfer injury were significantly increased(p<0.05).ConclusionsWJCs can change the microenvironment of the lesions of SCI by up-regulatingthe expression of anti-inflammatory factor IL-10, as well as GFAP, NSE and BDNF,while down-regulating pro-inflammatory factor like TNF-a and MCP-1, and furtherimprove the recovery of neural functional. These may be one of the mechanisms ofWJCs on reducing the spinal cord injury, promoting the functional and structuringrecovery of injured spinal cord of rats. |
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