The Differentiation Comparative Research Of Different Pubertal Rat Embryo And A Variety Of Animal Embryo’s Extraction Protein Induced The Hela Cell Apoptosis

From the perspective of developmental biology and tumor originated in the body’s normal stem cells and tumor cells is abnormal differentiation phenomenon, tumor should be a group of cells apoptosis and apoptosis occurred. Therefore, from the perspective of development can be fundamentally conquer cancer. Embryos in the process of the development of the embryo itself to synthetic differentiation factor (such as Nodal protein) guided embryonic cell differentiation of normal cells. Studies have shown that the differentiation factor can induce differentiation and apoptosis of tumor cells.Studies have shown that the animal cells extracted some protein to tumor cell differentiation and apoptosis. Other LABS and the lab, a large number of studies have shown that the embryo extract on inducing tumor cell apoptosis is universal. Guess in this lab, and prove that give play to the role of induction is of embryos to extract proteins. This embryonic coarse protein has the advantages of green, efficient, but it also has complicated composition, such as large dosage of faults.This laboratory by chicken embryos to extract protein, toad embryos protein proved: inducing tumor cell apoptosis as embryonic development, promote the differentiation of embryonic synthetic factor will decrease gradually. This experiment with mice embryos protein as inducers, further explore the conclusion. Selection of development for five days, ten days, fifteen days,20days of mouse embryos,4days of age mice and adult rat organs to extract protein, respectively. Protein concentrations were set to2mg/mL,4mg/mL and6mg/mL. These proteins induced Hela apoptosis, as embryonic development, embryo protein induced effect gradually reduced.The separation of proteins in the biology is a difficult problem. In order to improve the efficiency of inducing tumor cell apoptosis, this study selected the relatively efficient embryos protein; To explore two embryos proteins exert synergy common Hela induced apoptosis. With the fly embryo, embryo, frog guppy embryo, toad embryos to extract protein as inducers. Will fly embryos protein concentration is set to1mg/mL,2mg/mL,3mg/mL, repeating the induction experiments respectively. Other embryo proteins performed the same experiment. With optical microscope, fluorescence microscope and flow cytometry instrument detection means such as detection rate of tumor cell apoptosis. Found that fly embryos protein has the highest efficiency, other progressive decrease. These four protein combination of two mixed protein (1mg/mL, a total of2mg/mL) and four kinds of protein (1mg/mL) induced effect than alone, don’t have an advantage. Them with the concentration of2mg/mL of embryos protein induced effect than alone, the difference is huge. This shows that now no collaborative between them.