The Research Of Renal Cell Carcinoma On The Inhibition Of Signal Transduction In Using Of Tonic Heat Dampness Therapy And Chaifang

ObjectiveFiltering out the effective TCM prescriptions which could inhibit the growth ofrenal cell carcinoma according to the clinical treatment of renal cell carcinoma whichcommonly used in traditional Chinese medicine, and to observe the effective ofsuppression, explore the effective mechanism of tonic heat dampness therapy forkidney cancer disease, and prove TCM could provide effective and feasible treatmentfor kidney cancer diseases.MethodsReferencing the literature, CNKI June1979~March2013, which about thechinese medicine treatment for kidney cancer, researcher summarized193kind ofcommonly used traditional Chinese medicine as an initial screening specific drugthrough frequency analysis. And then Researcher soak and extract these initialscreening drugs in95%medical ethanol, and format the purified medicine after7days. Then prepare human786-0cell renal clear cell carcinoma in standard of100μg/ml drug concentration. Put specimen of786-0cell upside down under themicroscope and observe its morphology change. After48hours, filtering out the drugwhich have an definite disincentive impact on the kidney clear cell cancer786-0cellusing MTT colorimetric test. By using the orthogonal design method, elect the besteffective remedy and mix it in formula. Also in accordance with Western blotmethodology for observing the medical affection to the kidney clear cell cancer cells786-0expression. Explore the connection between the chinese medical pathogenicfactors of renal cell carcinoma occurs and growth signal transduction of786-0cell.Thereby, look for the most effective remedy for the treatment of kidney cell cancer. Results1The tonic heat dampness therapy to the cells786-0growth significantlysuppressed.2, The tonic heat dampness therapy to be effective in inhibiting cell786-0of thegrowth,the had strongest effect.The Suppression Ratio rising with the rising of drugconcentration and time. Inhibition of cell growth in a dose-dependent and time-dependent manner.3. Human renal clear cell carcinoma can be detected PI3K、AKT、NHkB proteinmolecules of the excess express.4. when IC50works on cells, The tonic heat dampness therapy can be effectiveregularly in all the protein expression level of PI3K、AKT、NHkB.Conclusion1The tonic heat dampness therapy and the other three part of therapy have adisincentive effect on the kidney cell cancer786–0cell. the Group works best，with a synergistic effect of tonic, clearing away damp and heat.2.kidney clear cell cancer786-0suppression with dose and time-dependent role.3. Through the performance counter, and confirmed that the kidney cancerlesions closely associated with asthenia of kidney, toxic heat, edema and PI3K、AKT、NHkB signal expression and enhanced transduction.4.The literature analysis and experimental results show that：kidney tumordiseases have deficiency of both qi and blood, toxic heat, risk.