Influence Of Bushen Zengmo Prescription On The Expression Of LIF In Endometrial Of COH Mice During Peri-inplantation

Objective:By observing the influence of Bushen Zengmo Prescription on theexpression of LIF in endometrial of Controlled ovarian hyperstimulation (COH)mice during peri-inplantation period, and discuss about the influence and mechanismof Bushen Zengmo Prescription to improve the receptivity of endometrium.Methods:50mice in accordance with the inclusive criteria were randomly dividedinto five groups: blank control group(A), model control group (B), the positivecontrol group (C)(aspirin group), the low dose of Bushen Zengmo Prescriptiongroup (D), the high dose of Bushen Zengmo Prescription group (E)，total of fivegroups. According to the requirements, the blank control group was treated withsaline solution by intraperitoneal injection, other groups were treated with propyleneammonia injection gonadotropin/urine gonadotropin/human chorionic gonadotropin(GnRHa+HMG+HCG)by intraperitoneal injection which were to build the controlledovarian hyperstimulation mouse model. at the same time, group A and group B weretreated with normal saline0.02ml/g to lavage; Group C, group D and group E weretreated with aspirin sodium carboxyl methyl cellulose solution, low-dose of BushenZengmo decoction, high dose of Bushen Zengmo decoction to lavage;2times/d,11days. And48hours after injection of HCG,Blank group since the vaginal smearcontinuous observation after48h after,then using immunohistochemical method tomeasure the expression of LIF protein in mice endometrial, using reversetranscription polymerase chain reaction (RT-PCR) to detect the expression ofendometrial LIFmRNA on Controlled ovarian hyperstimulation mice.Results：1.LIF in mouse endometrium during peri-inplantation period of mainexpression parts is the cavity epithelium and glandular epithelium, the glandularepithelium expression is rich, cavity epithelium expression is a little less,slightly expressed in the stroma.2.Each group of LIF protein expression: the expression of LIF protein in micecompared with the blank control group, model control group obviously decreased theexpression of LIF protein, the difference was statistically significant (P=0.012,P<0.05); Compared with model control group, the expression of LIF protein in mice’ssuper ovulation group were higher than that of the control group, the difference wasstatistically significant (P<0.05); And Compared with positive control group, thelow dose of Bushen Zengmo Prescription group, the expression of LIF protein in thehigh dose of Bushen Zengmo Prescription group was obviously increased, thedifference was statistically significant (P<0.05). And compared positive controlgroup with the low dose of Bushen Zengmo Prescription group, the expression ofLIF protein of two groups is similar, there was no statistically significantdifference(P=0.094, P>0.05).3.Each group of LIFmRNA express: compared with model control group, eachgroup of mice LIFmRNA expression increased, the difference was statisticallysignificant (P<0.05); the LIFmRNA expression of the high dose of Bushen ZengmoPrescription group was significantly higher than the positive control group, BushenZengmo Prescription’s low dose group,the difference was statistically significant(P<0.05); The LIFmRNA expression of Positive control group is similar to low dosegroup, there was no statistically significant difference (P=0.069,P>0.05).Its expre-ssion regulation and LIF protein expression rule during peri-inplantation period inthe lining of the uterus are basically identical.Conclusion:1.Bushen Zengmo Prescription can promote normal endometrial LIFprotein and mRNA expression, effectively improve the receptivity of endometriumon the Controlled ovarian hyperstimulation condition.2.Bushen Zengmo Prescription can improve the effect of the receptivity ofendometrium of the Controlled ovarian hyperstimulation in mice and its effect issuperior to that of aspirin.