| Hepatic fibrosis, which is caused by a variety of pathogenic factors, is liver damage andinflammatory. It is progressive, diffuse and fibrosis hyperplasia of connective tissue insideliver. It is pathological process that cause excessive deposition of extracellular matrix inliver. The milder is called hepatic fibrosis, and the worse is called hepatic cirrhosis thatpseudolobule and nodule have been formed by way of rebuilding hepatic lobule’s structure.Hepatic fibrosis is common pathological basis of all the chronic liver disease, and theintermediate link of hepatic cirrhosis what hepatic fibrosis will further develop into. Themain cause of hepatic fibrosis is chronic hepatitis B, moreover, some drugs and poisonssuch as ethanol, and carbon tetrachloride, some diseases such as viral hepatitis,schistosomiasis, autoimmune diseases, and others such as metabolic disorders, malnutrition,cholestasis, venous blood stasis can also lead to hepatic fibrosis.Objective: Based on the theory of Traditional Chinese Medicine and applying modernmethod to the animal experiment, this subject planed to study with the immunity hepaticfibrosis rat model. It was purpose to observe and research SNSJW high-dose group,mediate-dose group, low-dose group, and prevention group how to affect typeⅠ, Ⅲ,Ⅳ collagen, hyaluronic acid(HA), and α-smooth muscle actin(α-SMA) of the immunity rathepatic fibrosism. Compared with Colchicin and SNS, we could search advantage anddisadvantage between these groups in order to provide scientific basis for prevention andcure hepatic fibrosis. Through this study investigated the mechanism of antifibrotic actionand proper dose and the preventive action of SNSJW.Methods: The subject planed to study with the immunity female rats with hepaticfibrosis that was caused by pig serum.80female Wistar rats,of healthy, clean class,and120to130g weight, were randomly divided into8groups (normal group, pathologic modelgroup, SNS group, SNSJW low-dose group, SNSJW mediate-dose group, SNSJWhigh-dose group, SNSJW prevention group, and Colchicin contrast group), and10rats each group. Except normal group, all the other groups were injected with pig’s serum withoutinactivation into abdominal cavity, twice per week and0.5ml each time, persisting for10weeks. The prevention group was injected the stomach with the medicines18o’clockeveryday when models began to be made, the other treatment groups were dealed with justas the prevention groups5weeks later persisting for5weeks. All rats were taken foranesthesia by intraperitoneal injections with chloral hydrate at the end of10th week. Then,disset these rats,, collect blood from fabdominal aorta or blood biochemical tests, andremove the liver for pathological section.Results:(1)Liver tissue specimens: the liver tissue of pathological model group wasdark and red. The surface of liver was full of symmetrical spottedness. The therapeuticgroups: the color, texture, magnitude and surface of liver tissue were all improvedobviously compared with pathological model group.(2)SNSJE can decrease the content oftype Ⅰ, Ⅲ, Ⅳ collage, HA and α-SMA obviously and alleviate the degree of hepaticfibrosis. Compared treatment group with the pathologic model group, there was obviousdifference（P＜0.05）. The mediate-dose group was better than low-dose and high-dose（P＜0.05）,high-dose was better than low-dose, but there was no obvious difference（P＞0.05）.SNSJW prevention group was better than mediate-dose group and Colchic in contrastgroup, but there was no obvious difference（P＞0.05）.Betwwen SNSJW mediate-dosegroup and Colchicin group, however, SNSJW mediate-dose was better than Colchicingroup from positive expression of type Ⅰ, Ⅲ, Ⅳ collage, HA and α-SMA in the liver ofrats. Moreover, here was obvious difference between SNSJW mediate-dose group and SNSgroup（P＜0.05）.Conclusion: SNSJW could reduce the deposition of type Ⅰ, Ⅲ, Ⅳcollage, HA andα-SMA. It had a positive action of treatment and mediate-dose group had a better curativeeffect,the curative effect of the prevention group was best. |
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