| Objective:By establishing the rat model with chronic fatigue, we observe GuiluYishen formula particles on chronic fatigue rat general situation and behaviouralindicators (exhaustion swimming time, the rat tail suspension static time),observemicroscopic structure changes of Chronic fatigue rat skeletal muscle mitochondriaunder the electron microscope, detect the change of serum succinate dehydrogenase toexplore the pathogenesis of chronic fatigue syndrome;Observe Guilu Yishen formulaparticles on chronic fatigue rat skeletal muscle mitochondria microstructure and theinfluence of serum SDH to explore its action mechanism, and then provideexperimental evidence and theoretical support for the clinical application of GuiluYishen formula particles to treatment CFS.Methods:40SPF SD male rats,weight200±20gram, after adaptive feeding1week,werandomly selected16without modeling the remaining24carried modeling.weadopted forced exhaustion swimming,bound and clip tail composite factorsstimulation to construct the chronic fatigue rat model.After the the success ofmodeling, without modeling was randomly divided into two groups, namely,thenormal group and the normal control group; the modeling was randomly divided into3groups,respectively, the model group, the Desert cistanche kidney group and the GuiluYishen group.From the next day after the end of the modeling,on the base ofordinary breeding,the normal group and the model group were given saline gavage，the normal control group and the Guilu Yishen group were given Guilu Yishenformula particles suspension liquid gavage, the Desert cistanche kidney group wasgiven the Desert cistanche kidney particle suspension liquid gavage.Rats in eachgroup were administered1times/d, for14consecutive days.We measured the rat bodyweight, exhaustion swimming time and the rat tail suspension static time in the daybefore modeling,after modeling and after the last administration.After the end of theexperiment,we took the piece of right hind limb skeletal muscle of rats in each group,and observed microscopic structure of skeletal muscle mitochondria under theelectron microscope after abdominal cavity anesthesia.We separated the supernatantfrom the abdominal aortic blood,and then evaluated the serum SDH activity by ELISAmethod.We used IBM SPSS19.0statistical software to process the data.Results:(1) After modeling the end, each group of rats modeling grew slowly or evenreduced weight, reduced food intake and water consumption, squinting lazy move,loose stools, hair lost luster.Compared with the normal group,the weight,theexhaustion swimming time, and the rat tail suspension static time of the Normalcontrol group had no significant difference (P>0.05);Model group, the Guilu Yishengroup and the Desert cistanche kidney group rats weight loss, swimming exhaustiontime shortened and the rat tail suspension static time prolonged,and had significantdifference (P <0.05).After the administration, compared with the normal group,theexhaustion swimming time prolonged of the Normal control group had significantdifference,the Guilu Yishen group had no significant difference, the weight and the rattail suspension static time of the two groups had no significant difference (P>0.05);the Desert cistanche kidney group weight loss,and had significant difference (P<0.05),swimming exhaustion time shorten,the rat tail suspension static timeshortened,and had no significant difference (P>0.05);the model group weight loss,swimming exhaustion time shortened and the rat tail suspension static timeprolonged,and had significant difference (P <0.05). Compared with the Modelgroup,the Guilu Yishen group and the Desert cistanche kidney group weight gainsignificantly,swimming exhaustion time prolonged obviously, rat tail suspension timeshortened,and had significant difference (P <0.05).Compared with the Desertcistanche kidney group,the Guilu Yishen group swimming exhaustion time prolongedhad significant difference (P <0.05),and weight gain,the rat tail suspension timeshortened,and had no significant difference (P>0.05).(2)Model group rats skeletal muscle fibril arranged disorder,Z line,M line were fuzzyand disordered arrangement,the shape of mitochondria was irregular,and the volumewas smaller obviously than the normal group,most of the mitochondria appearedvacuoles,membrane dissolved and connect to each other.Compared with the modelgroup,the rat skeletal muscle myofibril of the Guilu Yishen group decreased and theDesert cistanche kidney group arranged more neatly,Z line and M line were more clearand tidy,the volume of mitochondrial was larger,a small amount of mitochondrialappeared vacuoles degeneration.Compared with the normal group,skeletal muscle fibril in rats of the normal control group arranged more neatly,Z line and M linewere more clearly and arranged more regular,mitochondrial structure was morecomplete and the volume of mitochondrial was larger.(3)Compared with the normal group,the Normal control group decreasedobviously,and have significant difference(P <0.05)；the Guilu Yishen group increasedmildly,and there was no significant difference(P>0.05)；the Desert cistanche kidneygroup and the Model group increased， and the difference was significant(P <0.05).Compared with the Model group,the serum SDH activity of the Desert cistanchekidney group and the Guilu Yishen group decreased,and the difference was significant(P <0.01).Compared with the Desert cistanche kidney group,the Guilu Yishen groupdecreased,and have significant difference (P <0.05).Conclusion:(1) This experiment uses the Non-virus infection in chronic stressmethod,namely forced exhaustion swimming,bound and clip tail composite factorsstimulation,to construct the chronic fatigue rat model.This method is operatedeasily,can simulate internal and external environment of resulting in humanfatigue,and can replicate the chronic fatigue state of human body.(2) Guilu Yishen formula particles can extend the chronic fatigue rat swimmingexhaustion time, shorten time of the rat tail suspension static time and protect theintegrity of the Mitochondria structure, reduce the activity of serum SDH.(3) The experimental results show that skeletal muscle and the structure of itsmitochondrial in rats with chronic fatigue are easy to damage, and not easy torecover, serum SDH activity increases significantly.Guilu Yishen formula particlescan enhance the stability of the skeletal muscle and its mitochondrialmembrane,promote the recovery of its damage,enhance mitochondrial function,andreduce serum SDH activity.It prompts Guilu Yishen formula particles can reach therole of fatigue resistance by protecting the structure of mitochondrial and enhancingits function,further explains the pathogenesis of chronic fatigue syndrome and theaction mechanism that Guilu Yishen formula particles treats this disease,and providesthe experimental basis for the clinical application that Guilu Yishen formula particlestreats chronic fatigue syndrome.(4)Guilu Yishen formula particles can be used as a preventive medication of CFS. |
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