| Objective: In this study, observation of Jianpi Qinghua Decoction on hepatitis Cvirus by in vitro (HCV) from human liver cancer cell line HepG2in apolipoprotein B(ApoB), very low density lipoprotein (VLDL), triglyceride (TG) effect, mechanism ofinterference analysis Jianpi Qinghua Decoction intervention scientific and objectiveanti HCV. Provides a new angle of view for Chinese medicine treatment of chronichepatitis C.Methords:(1)The purchase of HepG2cells and SPF40SD male rats, using themethod of randomized SD rats were divided into four groups: Chinese medicine group,western medicine group, combined group and blank control group, with10rats ineach group, three groups were given Jianpi Qinghua Decoction orally, interferonsubcutaneous injection of Gary Bhave Lin lavage Jianpi Qinghua Decoction, gastriclavage plus interferon subcutaneous injection of Gary Bhave Lin orally, the controlgroup was given saline gavage,10days after the rat serum, produced four groups ofrats serum.(2) The serum of patients with chronic hepatitis C, hepatitis C virus (HCV)infection in cultured HepG2cells.(3) Four groups of rats serum respectivelyintervention HCV HCV infected HepG2cells.(4) In hepatitis C virus (HCV) infectionand drug serum intervention,48h,96h,144h,192h cells and supernatant werecollected, by reverse transcription polymerase chain reaction (RT-PCR) detection ofHepG2cells in hepatitis C virus load, content changes of ApoB, VLDL, TG weredetected by ELISA in the supernatant from HepG2cells the. Through the analysis ofthe results of each detection mechanism Jianpi Qinghua Decoction interventioninterferon anti HCV.Results: HCV-RNA positive and negative strand RT-PCR method was used todetect cell and supernatant of positive, suggesting the model is successful. The resultsshow:(1) The influence of lipid in the supernatant from HepG2cells: before modelingthe blank group and the model is compared to control group ApoB, VLDL, TGcontents, three kinds of lipid in the significant difference between the two groups were, and the line graph shows three lipid compared with the blank group weredecreased in different degree the supernatant from HepG2cells, suggesting that thecontrol group after ApoB, hepatitis C virus infection in VLDL, TG content is blankgroup decreased. Serum ApoB, VLDL were measured after the intervention, thecontent of TG in48h,96h,144h in the different groups with no significant differencebetween P<0.05, P>0.05; in192h, there was significant difference between the fourgroups, and the traditional Chinese medicine group, combination group comparedwith the control group, P values were less than0.05, with statistical significance,while the western medicine group compared with the control group P>0.05, nostatistical significance; and the line graph shows four groups with the extension oftime were decreased in different degree, the control group decreased the most obvious,followed by traditional Chinese medicine group, western medicine group. Effects onApoB, VLDL indicated that the combined group, the content of TG was the mostobvious, followed by the traditional Chinese medicine group, western medicine grouphad no obvious influence.(2) Of HCV infection effect of HepG2cells in HCV-RNAtiter is hepatitis C virus load: the titer of HCV-RNA in144h,192h between the fourgroups there was significant difference in144h, and combined treatment group andtraditional Chinese medicine group, western medicine group and control group weresignificantly different between; traditional Chinese medicine group and Westernmedicine group, combined group in192h, there was significant difference betweencombined group and Western medicine group, the control group. The line graph showsthe Chinese medicine group, western medicine group, united group and control groupwere increased in varying degrees, of which the control group significantly increased;in Chinese medicine group and Western medicine group, combined group was thelowest. Comprehensive inspection and graph analysis combined with the greatestimpact on the HCV-RNA titer, followed by western medicine group, Chinese medicinegroup had no obvious influence.Conclusion:The experiment results show that (1) the control to reduce thecontent of ApoB, VLDL, TG, HepG2cell supernatant group after hepatitis C virusinfection, and the positive relation with time, and negatively related to hepatitis Cvirus load.(2) jpqhr can regulate the content of ApoB, VLDL, TG and HepG2insupernatant of hepatitis C virus infection.(3) the United Group Jianpi QinghuaDecoction plus interferon Gary Bhave Lin group not only for HepG2cells were ApoB,VLDL, TG content of the regulatory role, also has the obvious inhibitory effect on hepatitis C virus, and better than the western medicine group.(4) concluded: JianpiQinghua Decoction can improve the efficacy of interferon anti HCV, and itsmechanism may be related to improving lipid metabolism. |
PDF Full Text Request