Chinese Medicine Clinical Observation For The Treatment Of Oviduct Inflammatory Infertility And Pathology Of Model Rats And The Impact Of TNF-α、HSP60

Objects1. Through clinical observation and follow-up, the Chinese medicine tre atment of this blood inside and outside treatment oviduct inflammatory ob structive infertility TCM symptoms and physical signs before and after, t est indicators, etc., and to follow the pregnancy rate, objective evaluati on and treatment of party the clinical curative effect and security of th e oviduct inflammatory obstructive infertility.2. Through research and he party chronic infertility due to tubal phlog istic sex of rat model of pathological tissue morphology, the effect of o bjective evaluate the effect of treatment.3. The research and the previous party chronic tubal inflammatory obstr uctive infertility of rat model of cuhk rat serum tumor necrosis factor (TNF-a), heat shock protein60(HSP60) content, the influence of further ex plore its action mechanism.4. Good for clinical promotion for new medicines and proprietary Chines e medicine provide reliable objective basis for the development and utili zation.Methods1.The oviduct inflammatory infertility patients were randomly divided i nto a and32cases of party group, and the gentry and vernal enema group o f31cases, blank control group,32cases of the same period. And simple or al Chinese medicine treatment of party group, and he add warm enema group is oral Chinese medicine enema treatment,1month is1treatment course, stay for3months, blank control group no drug therapy. Treatment before and after careful observation and record the patients’clinical symptoms and signs, the uterus fallopian tube ultrasonic imaging results, inspecti on index and its adverse reactions and follow-up each other pregnancy rat e, were followed up for half a year, to take their own group before and a fter the contrast and comparison method to analyze patients between chang e and statistical analysis.2. SPF60SD female rats,8-12weeks of age, weight,180-200g, provided by experimental animal surveillance in guangdong province. Animal qualifi ed number:SCXK (guangdong),2008-0002. The whole experiment process using natural water and normal feeding animals. Randomly divided into and of hig h, medium and low dose group, the building group, the blank group. Which, in addition to the12only as blank group,48for the rest of the model grou p.Use "reproductive tract mixed bacteria inoculation" induced chronic tub al experimental animal model of rats,2weeks after the test model results. To be made after the success of the module, respectively of low, medium a nd high dose and to fill the stomach, he made module on the same dose of blank group and normal saline lavage, continuous lavage for15days. In15days will kill rats, careful observation to the naked eye bilateral fallo pian tube shape and inflammation in rats, to return the rat serum and bil ateral fallopian tube specimens histologic examination. Using enzyme-linke d adsorption test (ELISA) determination of rat serum TNF alpha, HSP60lev els. Results1.And he party, and he add warm enema group, blank control group total effective rate was78.13%,87.10%,15.63%respectively, and the gentry g roup, and he add warm enema group compared with blank control group was s tatistically difference (P<0.05), but and he party between group and verna1enema with and he is no statistical significance (P>0.05).2. And he party, and he add warm enema group, blank control group, the pregnancy rate was28.13%,41.93%and6.25%respectively, and the gentry party, and he add warm enema group compared with blank control group the pregnancy rate was statistically difference (P<0.05), but and he party gr oup and the gentry party warm enema between two groups has no statistical significance (P>0.05). 3. The building of rats after2weeks, random samples showed bilateral fallopian tube hyperemia oedema, different degrees of wall thickening of the lumen, and basin wall mild adhesion, lumen obstruction, different lev el using the methylene blue solution for bilateral hydrotubation tip or b ilateral fallopian tube jammed far and near end of different degree of ob struction. Use reproductive tract inoculation copy "mixed bacteria" inflam matory obstructive infertility in rats model of success.4. By ELISA method to detect, and of high, medium and low group compare d with model group, both model was reduced after rat serum tumor necrosis factor (TNF-a), heat shock protein60(HSP60) content, among them, and o f high, middle dose group, respectively, compared with pure between model group was statistically difference (P<0.05), it indicates and of high, m iddle dose group of oviduct inflammatory infertility in serum of rat TNF-α、HSP60level has certain inhibitory effect.Conclusions1. Traditional Chinese medicine (TCM) and the party he obviously reduce the patient’s clinical symptoms and signs, improve its clinical effectiv eness and pregnancy rate, and he combined with external treatment vernal enema, further improve the clinical curative effect. So, this Chinese tradi tional medicine of blood and he combined with external treatment vernal e nema in treating oviduct inflammatory infertility method is worthy of cli nical promotion.2."the genital tract mixed bacteria inoculation method" can be copied successfully infertility due to tubal inflammatory model in rats.3. Experiment proof and the gentry in the oviduct inflammatory obstruct ive fallopian tubes in the model rats hyperemia edema and inflammation, s uch as in the thickness of the tube cavity are improved to varying degree s, is the ideal treatment effect.4.And he’s high, medium and low dose group were different degree to re duce the oviduct inflammatory obstructive model rats serum TNF-α、HSP60le vels.