The Irritation Of Rabbit’s Ocular Surface By Total Saponins Of Panax Notognseng Eye Drops

Objective:give total saponins of panax notognseng eye drops on normal rabbit eyes, establish a irritation index measure of total saponins of panax notognseng eye drops on the rabbit eye, to investigate the irritation reaction of total saponins (PNS) application on the local of rabbit eyes, confirm the safety of local application of total saponins of panax notognseng eye drops in rabbit eyes.Method:Select healthy40adult New Zealand white rabbits (80eyes), divided into blank control group,1%concentration group,2%concentration group,3%concentration group, each group10rabbits (20eyes).Check each rabbit eyes with fluorescein sodium dilution24hours before experiment, eye irritation symptoms, corneal and conjunctival defect injury in rabbit excluded from the experiment, the qualified rabbits for experiment. Method one:To give the total saponins of panax notognseng eye drops on the right eye of each rabbit in treatment, of which1%concentration group saponins of panax notognseng eye drops by1%,2%concentration group was2%,3%concentration group was3%, control group given the same amount of reagent blank, trace Straw absorb total saponin of panax notognseng eye drops agents were administered, conjunctival sac, a2drop, every time when administered oppression of nasolacrimal duct, and passive close rabbit eyelid in5seconds, recording10minutes of rabbits to observe and compare the blink of rabbits. Then by using SPSS17software for data rank sum test and Manny-whitney U test.Method two:To give the total saponins of panax notognseng eye drops on the left eye of each rabbit in treatment, of which1%concentration group saponins of panax notognseng eye drops by1%,2%concentration group was2%,3%concentration group was3%, control group given the same amount of reagent blank, administered3times daily, at intervals of4hours, continuous administration for7days, before administration and after the last dose of1,2,4,24,48,72h eye examination every day, if within72hours has no irritation, the end of the trial; If there is a slight stimulation, observation period is extended to the last7days after administration; if the moderate irritation, observation period is extended to the last14days after administration; if the intensity of stimulation, the observation period was extended to last up to21days after administration. And observe the conjunctiva, cornea and iris irritation reaction by slit lamp, according to eye irritation score record score of eye reaction. Then by using SPSS17software for data rank sum test and X2test (four exact test) test. Through the above two methods to measure the safety of total saponins of local treatment of bleeding in three seven rabbit eyes. Then by using SPSS17software for data rank sum test and X2test (four exact test) test. Through the above two methods to measure the safety of total saponins of local treatment of bleeding in three seven rabbit eyes.Results:1. Blink of an eye as a criterion:Each medication group (1%concentration group,2%concentration group,3%concentration group) blink without significant difference from the control group; each blink without statistical significance (P>0.05). each blink without statistical significance (P>0.05). Two two other comparative results show that:the effects of low concentration and the blank control group, middle concentration group and blank control group, high concentration group and blank control group, low concentration group and high concentration group, no significant difference (P>0.008); in the low concentration and concentration group, middle concentration group and high concentration group, no significant difference (P>0.008).2. The index of the rabbit cornea, iris, conjunctival irritation score results as a criterion:1%concentration group,2%concentration group,3%concentration group and blank control group had no statistical significance (P>0.05);1%concentration group,2%concentration group,3%groups had no statistical difference between the concentration of stimulus response (P>0.05).Conclusion:In the treatment of PNS eye drops in rabbit subconjunctival hemorrhage, were observed with the blink of an eye, corneal, conjunctival reaction, the rabbit iris, and secretion confirmed local treatment of total saponins of three seven eyes in rabbit subconjunctival hemorrhage is safe and feasible.