Identification Of Differentially Expressed MicroRNAs In Peripheral Blood T-lymphocytes After UVB Irradiation Of SLE Patients

Objective To explore the pathogenesis of systemic lupus erythematosus (SLE) after UltravioletB(UVB)irradiationthroughscreeningdifferentially expressed microRNAs of peripheral blood T-lymphocytes of patients with SLE.Methods Theperipheral blood of25cases with SLE patient and25normal controls were collected, and the peripheral T-lymphocytes were isolated and then divided into UVA irradiationgroup, UVB irradiation group and blank control group. Total RNA were extracted from the mixture of individual samples in each group and then an inter-group chip analysis was conducted by using Affymetrix miRNA chip. Real-time Quantitative PCR (RtQ-PCR) detection system was employed to verify the test results of UVB irradiationgroup miRNA.Results The inter-group chip analysis by using Affymetrix miRNA chip showed that1. The following13miRNAs screening out from the peripheral blood samples of SLE patients after UVB irradiation were miR-106b-5p, miR-129-5p, miR-125b-5p, miR-125a-5p, miR-96, miR-1909, miR-1290, miR-26a, miR-3180, miR-3180-3p, miR-1202, miR-3937and miR-4800-3p respectively.2. The results of13miRNAs showed that compared with normal persons, the miR-106b-5p expression levels of SLE patients groupwas higher, compared with patients without irradiation group, the expression level of SLE patients with UVB irradiation group was lower.The expression level of normal controls with UVB irradiation groupwas higher than that of without irradiation group. The results of the following12miRNA were identical.The expression levels of SLE patients were lower than that of normal persons.Compared with patients without irradiation group, the expression levels of SLE patients with UVB irradiation group were higher.The expression levels of normal controls with UVB irradiation group was higher than that of without irradiation group.3.The qPCRof miR-106b-5p, miR-129-5p and miR-125b-5p results indicated that A. The miR-106b-5p expression of SLE patients washigher than that of normal controls. Compared with SLE patients without irradiation group, patients with UVB irradiationgoup demonstrated higher miR-106b-5p expression.There was no differenceof miR-106b-5pexpression between normal persons with UVB irradiation and without. B. ThemiR-129-5p expression of SLE patients waslower than that of normal controls. There was no difference of miR-129-5p between SLE patients with UVB irradiation and withoutirradiation group.The miR-129-5p expression level of normal controls with UVB irradiation was lower than that of without irradiation.C. The miR-125b-5expression of SLE patients waslower than that of normal controls. Compared with SLE patients without irradiation, patients with UVB irradiation demonstrated higher miR-125b-5expression.There was no difference in miR-125b-5expression between normal persons with UVB irradiation and without.Conclusions Thirteen different miRNA expressions were screened out after the UVB irradiation ofperipheral blood T-lymphocytes in patientswith SLE. UVB irradiation might cause different expression of up or down among miRNAs. The study shows that UVB irradiation led to the significant up-regulations of miR-106b-5p and miR-125b-5p by qPCR. UVB irradiation may change the expression levels of miRNAs and associate witht the pathogenesis of SLE. To study the expression of miRNAs may providing a new directions for its pathogenesis and treatment.