The Research Of Plasma Circulation MicroRNAs Expression Profile Of MMA

Background and objectiveMethylmalonic acidemia is an organic acidemia with autosomal recessiveheredity which to be claimed the most common disorder of genetic organic acidmetabolism. Our research detected the expression level of microRNAs of patientsplasma circulation with microarray gene chip and analyzed differential expression ofmicroRNAs, meanwhile, explored the expression rule of plasma circulationmicroRNAs in MMA pathogenesis primarily and aimed at finding the representationby microRNAs of MMA new biomarkers and therapeutic evaluation index.Materials and methodsTo select10patients of vitamin B12valid of late onset type who were diagnosedin the Third Affiliated Hospital of Zhengzhou University from August,2011toJune,2013as MMA group,6males,4females, mean age13.0±3.5years old; All ofthem were tested methylmalonic acid analysis value of blood and urine bygas-chromatography mass spectrometry. To select8healthy patient relatives whohaven’t any diseases as control group,4males,4females, mean age12.6±2.1years old.We filed plasma and extracted microRNAs routinely,and evaluated samples qualitywith real-time quantitative PCR (real-time PCR) method,at the same time, detected the expression level of microRNAs of each group plasma circulation with microarraygene chip.Results1.The results of GC/MS presents the urine methylmalonic acid zoom ratio is2360.532±1589.506of MMA group before treatment, while the urine methylmalonicacid zoom ratio decreased variantly is624.540±420.946of all the patients,bothgroups are different significantly（P<0.01）.The urine methylmalonic acid zoom ratioof control group is8.284±2.615, less than100,compared with MMA group beforeand after treatment,the difference is remarkably（P<0.01）.2.The results of Real-time PCR shows hsa-miR-16and hsa-miR-192Ct number ofeach group samples plasma in reference range,melting curve was normal,whichexplained the RNA extracted from plasma samples conformed the requirement ofexperiment and can be delivered to downstream chip experiment.3. The chip statistical analysis suggests129expressions of microRNAs both controlgroup and MMA group before treatment are different significantly (P<0.01).Compared with control group,there are19raises and63reductions of microRNAs ofMMA group before treatment.4. The chip statistical analysis suggests183expressions of microRNAs both controlgroup and MMA group after treatment are different remarkably（P<0.01）. Comparedwith control group, there are37raises and88reductions of microRNAs of MMAgroup after treatment.5. The chip statistical analysis presents127expressions of microRNAs of MMAgroup before and after treatment are different strongly（P<0.01）. Compared withbefore treatment, there are2raises and71reductions of microRNAs of MMA groupafter treatment.Conclusion:1. There are several abnormal expressions of plasma circulation microRNAs onpatients with methylmalonic acidemia2.The plasma circulation microRNAs will be biomarkers of methylmalonic acidemia.