Correlation Research On Phosphodiesterase4D Gene Po-Lymorphism And Ischemic Stroke Of The Wa Nationality In Yunnan China

Objective:The current study aims to observe the mutations of SNPs rs966221and rs918592of PDE4D gene in Wa population from Lincang area. Combined with research objects’ hypertension, diabetes, smoking history, LDL levels and other risk factors for analysis of risk factors. We further compare the genotypic differences between the normal population and stroke patients, as well as the susceptibility to ischemic strokeMethods:A case-control study from4Lincang local hospitals was done comprising52Wa people with ischemic stroke,54Han people with ischemic stroke and55Wa people as healthy controls. SNPs of rs966221and rs918592in PDE4D were detected with direct sequencing of PCR. products. Logistic regression was used for removing confounding factors. The differences of genotype distribution and allele frequency among groups were compared. And the correlation between rs966221, rs918592sites and the incidence of ischemic stroke in Wa population was also evaluated.Results:(1) When compared the stroke patients from Wa population, with the healthy controls or the stroke patients from Han pupulation, no significant differences were observed in gender composition (P>0.05) or age distribution (F=1.463, P>0.05, pairwise comparisons P>0.05). Baseline conventional vascular risk factors were compared between the stroke patients in Wa population and Han population, including hypertension (χ2=4.932, P>0.05), diabetes (χ2=0.448, P>0.05), smoking (χ2=1.205, P>0.05), LDL level (t=1.080, P>0.05) and some others, no significant differences were observed between these groups; while the risk factors were more common among patients compared with controls in Wa population, with hypertension (χ2=4.665, P<0.05) and LDL level (t=8.145, P<0.05) significantly higer in the patients group. But other risk factors including diabetes mellitus (χ2=0.149, P>0.05), smoking (χ2=0.003, P>0.05), etc. showed no such differences. In this study, the rs966221and rs918592genotype frequency distributions were calculated following the Hardy-weinberg equilibrium principle (P>0.05). rs966221genotype distributions showed no significant difference between stroke patients from Wa population and Han population (χ2=1.029, P>0.05), as well as rs966221allele frequencies (P>0.05). When compared the stroke patients of Wa population to the healthy controls, no significant difference was observed in the genotype distributions (χ2=5.335, P>0.05). However, statistically significant allele frequency differences was observed between these2groups (χ2=6.644, P<0.05). The logistic regression analysis showed that the incidence of ischemic stroke in Wa population with C allele was3.376times more than the people without C allele (OR=3.376,95%CI=1.075-7.601, P=0.037<0.05). rs918592genotype distributions in stroke patients from Wa population showed no significant differences compared with the stroke patients from Han population (χ2=0.631, P>0.05). There were also no significant differences of genotype distributions in stroke patients from Wa population and healthy controls (χ2=2.195, P>0.05). Moreover, no significant differences in allele frequencies were observed among these three groups (P>0.05). By binary logistic regression analysis to remove confounding factors, rs918592might not be associated with the incidence of ischemic stroke in Wa population (P>0.05). Conclusions:1rs966221, rs918592genotype distributions showed no significant differences between ischemic stroke patients in Wa population and the local Han population.2rs966221allele frequencies showed significant differences between the healthy controls and ischemic stroke in Wa population. Among all, the C allele frequency was significantly higher in the patients. After the analysis of risk factors, C allele in rs966221might be a risk factor for ischemic stroke of Wa population in Lincang area.3rs918592allele frequencies showed no significant differences between stroke patients of Wa population and healthy controls, which indicated that rs918592site might be unrelated to the incidence of ischemic stroke for the local Wa population.4The higher LDL levels increase the risk of ischemic stroke for the local Wa population.5. C-G haplotype consisting of rs96621and rs918592in PDE4D genes, which is a potential genetic risk factor for ischemic stroke, T-G haplotype plays a protective role in the course of the disease of Wa nationality in Lincang, Yunnan province probably.