Roles Of FOXA1Transcription Factor In Breast Cancer Cell Proliferation

Tumor gene therapy refers to leading target gene with therapy function into target cells to acquire specific function to execute or mediate killing or controlling effect on tumor cell,and then to achieve therapeutic purpose. Looking for therapeutic target genes which can heal various malignant tumors has become a hot topic in recent years. The transcription factor FOXA1, which belongs to the FOX transcription factor family, has been discovered in previous studies that FOXA1took part in regulating such physiological and pathological process as cell proliferation and differentiation, organ’s development, embryogenesis, and also participates in the development and progression of tumors. In tumor cells FOXAlmay be involved in cell cycle regulation, thus affecting cell proliferation rate. Based on these previous studies, the present paper focused on the effect of FOXA1on the proliferation of breast cancer cell, and explored its molecular mechanisms of regulating cell proliferation.In this paper, breast cancer cell line MCF-7and ZR-75-30were chosen to be subjects; lentivirus mediating ShRNA-FOXA1was used to infect breast cancer cell line MCF-7with endogenous high expression of FOXA1, wiping out expression of FOXA1with specificity; at the same time, lentivirus of FOXA1with high expression was used to infect breast cancer cell line ZR-75-30with low endogenous expression of FOXA1; WB and SQRT-PCR methods were applied to detect expression level of FOXA1and inspect the expression changes of potential target gene P21, P27of FOXA1Results showed that there was a positive correlation between expression levels of FOXA1and P21, P27, which indicated that FOXA1may regulate cyclin-dependent kinase to inhibit gene transcription of P21and p27, and involved in cell cycle regulation. Then, we inspected the effect of FOXA1on breast cancer cells’ proliferation:through drawing the curve of cell growth, and detecting of flow cytometry we discovered that FOXA1of high expression could repress the cell proliferation, and stopped cell cycle to G0/G1phase.