| Objective Background:To investigate the pathogenic effect of Th22, Th17cells in patients with acute myocardial infarction, we detected the expression of Th22, Th17cells in coronary artery thrombosis and peripheral blood of AMI patients and the peripheral blood of stable angina patients and control group of healthy people, and examined the concentration of plasma interleukin-22(IL-22) and interleukin-17(IL-17) in correspo-nding individuals. We studied the relation between cells, their cytokines and the occurrence of AMI, in order to further illuminate the possible association between Th22, Thl7cells and the pathogenesis of acute myocardial infarction and offer a new auxiliary index for diagnosis of acute myocardial infarction in the future.Materials and Methods:A total of43patients with coronary heart disease confirmed by coronary angiography, including28AMI patients (all of them are ST-segment elevation myocardial infarction) and15SA patients, and the other15cases of normal coronary angiography as a healthy controls (HC) group. Enrollment were recorded with age, gender, drinking, smoking, and other related diseases. The serum troponin T (TnT) of participants tested by chemical immune luminescence determination was also recorded. We took10cases of infarction related artery thrombosis by using the coronary artery thrombus suction device and collected the fasting blood samples of each group by disposable vacuum blood sampling device. The time for acute myocardial infarction patients of taking peripheral blood were within12hours and2weeks respectively. The proportion of Th22, Th17cells in CD4+T cells were detected by using flow cytometry instrument staining method. The expression level of IL-22and IL-17in peripheral blood plasma of each group were tested by enzyme-linked immunosorbent assay (ELISA).Results:(1) The proportion of Th22(5.84±1.16%), Th17(4.86±1.03%) cells in coronary artery thrombus of AMI were significantly higher than the rate of Th22(3.26±0.95%), Th17(2.95±0.94%) cells in peripheral blood of the onset of AMI within12hours(all p<0.05)(2) The frequencies of Th22(3.26±0.95%), Th17(2.95±0.94%) cells in peripheral blood of the onset of AMI within12hours were increased compared with AMI onset of2weeks (1.64±0.46%,1.93±0.50)、 SAP(0.92±0.26%,1.59±0.49%)and HC(0.84±0.19%,1.35±0.32%)(all p<0.05).Also, the ratio of Th22, Thl7in AMI onset of2weeks were significantly higher than that of SAP and HC group (P<0.01). There was no statistically significant difference between SAP and HC group。(3) The concentration of plasma IL-22(33.74±7.25) pg/ml and IL-17(24.95±8.86pg/ml)in AMI were significantly elevated compared with SAP(26.95±3.19pg/ml,18.86±5.65pg/ml)andHC(24.17±2.55pg/ml,14.63±2.57pg/ml) group(all p<0.05) and no marked difference was found between SAP and HC.(4) There was a positive correlation between Th22cells and Th17cells in coronary artery thrombus (r=0.981, p<0.01), peripheral blood of the onset of AMI within12hours(r=0.517, p<0.01) and peripheral blood of the onset of AMI2weeks(r=0.429, p<0.05). Similarly, a positive correlation was also found between serum troponin T (TnT) and Th22cells(r=0.863, p<0.01), Th17cells (r=0.405, p<0.05) in peripheral blood of the onset of AMI within12hours. Also, the percentage of Th22cells in peripheral blood of the onset of AMI within12hours and IL-22(r=0.771, p<0.01), the ratio of Thl7cells and IL-17(r=0.627, p<0.01) all have significant positive correlation.Conclusion:(1) The local immune inflammation in the coronary artery thrombus of Th22, Thl7 cells in AMI was more strongly than systemic inflammatory response.(2) Th22,Th17cells and their cytokines are closely associated with the onset of AMI, so they can be considered as new auxiliary diagnostic index to AMI. |
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