BNIP3Suppresses PTEN-induced Kinase1(PINK1)Null Mutant Phenotypes In Drosophila

Parkinson’s disease is a common neurodegenerative disorder characterized by motor disturbances and dopaminergic neurodegeneration, its incidence is second to Alzheimer’s disease. A growing body of evidence directly links mitochondrial autophagy and apoptosis to Parkinson’s diseases. BNIP3is classified as pro-apoptotic member of BH3-only sub-family. Apart from its role in cell death, BNIP3is also implicated in the induction of mitochondrial autophagy. The existing body of knowledge suggests that maybe BNIP3is associated with Parkinson’s disease.Objective:To test whether BNIP3is associated with Parkinson’s disease.Methods:First we obtain BNIP3transgenic Drosophila UAS-BNIP3WT by micro-injection.Then using the GAL4-UAS system specific over express BNIP3in different tissues in Parkinson disease drosophila model (PINK1null mutant drosophila). Finally, we observe and record the difference between fruit flies.Results:We have found that overexpress BNIP3in indirect flight muscles can suppress morphological defects induced by PINK1 inactivation in Drosophila. Moreover, BNIP3restores mitochondrial dysfunction (ATP levels and indirect flight muscles morphology) caused by PINK1inactivation in Drosophila. Furthermore, BNIP3restores dopamine production in brains of PINK1null mutant flies.Conclusion:BNIP3Suppresses PTEN-induced Kinase1(PINK1) Null Mutant Phenotypes in Drosophila, this confirms that BNIP3is linked to Parkinson’s disease.