Expression Of Oct4in Breast Cancer Tissue And Its Effect On Cell Invasion Of Breast Cancer

Purpose：To examine the expression level of Oct4in breast cancertissue and further investigate its effect on cell invasion of breast cancer.Methed：Western blot analysis was used to detect the protein level ofOct4in27paired breast cancer tissue and adjacent non-tumoral breasttissue. Oct4expression in breast cancer cells was knockdown bylentivirus-mediated shRNA interference technology. The effect of oct4silencing on the cell invasion was determined by wound-healing assay andcell invasion assay. Four EMT markers (E-cadhrin, alpha-catenin, vimentinand N-cadherin) were analyzed by western blot analysis.Result：The results demonstrated that74%(20/27) patients showedelevated Oct4expression in breast cancer tissue compared with that inadjacent non-tumoral breast tissue. Lentivirus-mediated shRNAsignificantly suppressed Oct4expression. Gene silencing of oct4markedlyinhibited invasion of breast cancer cells. Knockdown of oct4in breastcancer cells induced the expression of epithelial markers E-cadherin andα-catenin that was accompanied by a concomitant reduction of mesenchymal marker N-cadherin and vimentin. These finding suggestedthat Oct4expression was upregualted in breast cancer tissue. Genesilencing of oct4inhibited cell invasion of breast cancer through regulatingEMT.