Correlation Between CHGA Genetic Polymorphism And Prognosis Of Critically Ill Patients

Objective: To investigate the correlation between single nucleotidepolymorphism in the promoter region or the7thexon of chromogranin A(CHGA) and prognosis of critically ill patients.Methods: Totally294critically ill patients consecutively admitted toour ICU were recruited. The-89/-415/-462locus in the promoter region andthe+9559/+9578/+9590/+9611in the7thexon of CHGA geneticpolymorphisms were determined the by polymerase chain reaction andDNA sequencing technology, subsquently correlation between genotypeand clinical characteristics of patients were analyzed.Results:â‘ There was no mutant type to be determined in theCHGA-89/+9578/+9590SNPs, the rest4SNPs are polymorphismdistribution, but no significant difference in the minor allele frequency(MAF) of CHGA-415/-462/+9559/+9611genetic polymorphism betweenparticipants of this study and the healthy people in Asia.â‘¡The CHGA-415T/C MAF of death group was significantly higher than that of survivalgroup (MAF0.378and0.292respectively, P=0.046), but there was nosignificant difference in the rest6CHGA SNPs genetic polymorphisms between these two groups.â‘¢Survival analysis showed that there weresignificant differences between CHGA-415C/T mutation group (includingTC and CC genotypes) and wild group (TT genotype)(Log rank=7.331;P=0.007). The mortality in mutant group was significantly higher than thatin wild group (0.325and0.191, respectively; P=0.010). Binary logisticanalysis showed that CHGA-415T/C polymorphism was an independentrisk factor for the mortality of critically ill patients (OR=2.055,95%CI=1.051-4.019, P=0.035).Conclusions: Critically ill patients with CHGA-415C/T mutantgenotype display higher30d mortality than wild genotype group.Furthermore the death group had higher MAF than survival group. TheCHGA-415T/C polymorphism could be an independent risk factor for30d mortality in critically ill patients.